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Anti cd68 mab clone fa 11

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Anti-CD68 mAb (clone FA-11) is a monoclonal antibody that recognizes the CD68 antigen, a glycoprotein expressed on the lysosomal membrane of monocytes, macrophages, and certain other cells. This antibody can be used to detect and identify cells of the monocyte/macrophage lineage in various applications.

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Lab products found in correlation

Deadend fluorometric tunel system, by Promega (2 mentions) Oct compound, by Gentaur (2 mentions) Anti ki67 pe conjugated mab clone 16a8, by BioLegend (2 mentions) Alexa fluor 488 conjugated anti rat antibody, by Thermo Fisher Scientific (2 mentions) Oil red o, by Merck Group (2 mentions)

Spelling variants of this product

Anti-CD68 (77 citations) CD68 (FA-11; (14 citations) FA-11 (10 citations) Anti-CD68 (clone FA-11, (3 citations) Clone FA-11 (3 citations) CD68 (clone FA-11) (2 citations)

2 protocols using anti cd68 mab clone fa 11

1

Aortic Plaque Evaluation in Mice

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Mice were sacrificed and slowly perfused with 10ml of ice-cold PBS. The hearts and aortas were carefully excised and fixed in 4% paraformaldehyde containing 30% sucrose. The aortas were stained with Oil Red O (Sigma-O0625) to evaluate plaque neutral lipid content. The hearts were embedded in OCT compound (Gentaur) and stored at −80°C before analysis. 10μm cryosections of the aortic sinus were prepared. Oil Red O staining was used to detect neutral lipid content in the plaque combined with a haematoxylin/eosin staining to analyse tissue architecture. Plaque macrophages were visualized using purified anti-CD68 mAb (clone FA-11, AbD Serotec). Anti-rat Alexa Fluor 488-conjugated antibody (A-11006, Life technologies) was used for detection of CD68 staining. For analysis of plaque macrophage proliferation, anti-Ki67 PE conjugated mAb (clone 16A8, BioLegend) was used. Nuclei were revealed with DAPI counterstaining (2μg/ml). TUNEL staining was performed using the DeadEnd™ Fluorometric TUNEL System (Promega). Plaque area quantification were measured with ImageJ software.
Merlin J., Ivanov S., Dumont A., Sergushichev A., Gall J., Stunault M., Ayrault M., Vaillant N., Castiglione A., Swain A., Orange F., Gallerand A., Berton T., Martin J.C., Carobbio S., Masson J., Gaisler-Salomon I., Maechler P., Rayport S., Sluimer J.C., Biessen E.A., Guinamard R.R., Gautier E.L., Thorp E.B., Artyomov M.N, & Yvan-Charvet L. (2021). Non-canonical glutamine transamination sustains efferocytosis by coupling redox buffering to oxidative phosphorylation. Nature metabolism, 3(10), 1313-1326.
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2

Aortic Plaque Evaluation in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mice were sacrificed and slowly perfused with 10ml of ice-cold PBS. The hearts and aortas were carefully excised and fixed in 4% paraformaldehyde containing 30% sucrose. The aortas were stained with Oil Red O (Sigma-O0625) to evaluate plaque neutral lipid content. The hearts were embedded in OCT compound (Gentaur) and stored at −80°C before analysis. 10μm cryosections of the aortic sinus were prepared. Oil Red O staining was used to detect neutral lipid content in the plaque combined with a haematoxylin/eosin staining to analyse tissue architecture. Plaque macrophages were visualized using purified anti-CD68 mAb (clone FA-11, AbD Serotec). Anti-rat Alexa Fluor 488-conjugated antibody (A-11006, Life technologies) was used for detection of CD68 staining. For analysis of plaque macrophage proliferation, anti-Ki67 PE conjugated mAb (clone 16A8, BioLegend) was used. Nuclei were revealed with DAPI counterstaining (2μg/ml). TUNEL staining was performed using the DeadEnd™ Fluorometric TUNEL System (Promega). Plaque area quantification were measured with ImageJ software.
Merlin J., Ivanov S., Dumont A., Sergushichev A., Gall J., Stunault M., Ayrault M., Vaillant N., Castiglione A., Swain A., Orange F., Gallerand A., Berton T., Martin J.C., Carobbio S., Masson J., Gaisler-Salomon I., Maechler P., Rayport S., Sluimer J.C., Biessen E.A., Guinamard R.R., Gautier E.L., Thorp E.B., Artyomov M.N, & Yvan-Charvet L. (2021). Non-canonical glutamine transamination sustains efferocytosis by coupling redox buffering to oxidative phosphorylation. Nature metabolism, 3(10), 1313-1326.
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