The blood samples were collected to observe changes in the levels of IL-6, TNF-α, S100β, NSE and MDA before inducing anesthesia (T1), at the end of surgery (T2) and three days after the end of surgery (T3). Blood samples (5 ml) were allowed to clot for 2 h at room temperature and were then centrifuged for 15 min at 2000×g at 4°C. The serum fraction was removed and stored at −80°C for further analysis. The levels of IL-6, TNF-α, S100β and NSE were measured using an Enzyme-linked immunosorbent assay (ELISA) kit (Neobioscience Technology Company, Beijing, China) according to the manufacturer’s protocol. MDA concentrations were determined using enzymatic methods following the manufacturer’s instructions (Jiancheng Biologic Project Company, Nanjing, China). All study personnel were blinded to the results of the laboratory analysis.
Enzyme linked immunosorbent assay elisa kit
Manufactured by Neobioscience
Sourced in China
The Enzyme-linked immunosorbent assay (ELISA) kit is a laboratory tool used to detect and quantify specific proteins or other analytes in a sample. It utilizes antibodies and enzyme-mediated color changes to measure the presence and concentration of the target molecule.
Automatically generated - may contain errors
Lab products found in correlation
Sodium hydroxide (naoh), by Chron Chemicals (2 mentions)
Superoxide dismutase sod kit, by Nanjing Jiancheng (1 mentions)
Malondialdehyde mda kit, by Nanjing Jiancheng (1 mentions)
Anti β actin antibody, by Boster Bio (1 mentions)
Rabbit anti tlr4 polyclonal antibody, by Proteintech (1 mentions)
Bca protein quantification kit, by Beyotime (1 mentions)
Dual luciferase reporter gene assay kit, by Promega (1 mentions)
Superreal premix kit, by Tiangen Biotech (1 mentions)
Streptozotocin (stz), by Merck Group (1 mentions)
Dextran standard, by Merck Group (1 mentions)
Xylose, by Merck Group (1 mentions)
Mannose, by Merck Group (1 mentions)
Arabinose, by Merck Group (1 mentions)
Rhamnose, by Merck Group (1 mentions)
Fucose, by Merck Group (1 mentions)
Galactose, by Merck Group (1 mentions)
Dulbecco s modified eagle medium dmem, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Penicillin, by Merck Group (1 mentions)
Streptomycin, by Merck Group (1 mentions)
6 protocols using enzyme linked immunosorbent assay elisa kit
1
Perioperative Biomarker Dynamics
2
Hair-based Antioxidant Evaluation
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Human hairs were collected from local barber shops in Chongqing China. Thioglycolic acid (TGA), sodium dodecyl sulphate (SDS), hydrogen peroxide (H2O2), hydrochloric acid and sodium hydroxide were purchased From Kelong Chemical Reagent Co., Ltd, China. Malondialdehyde (MDA) kit, Gycogen (PAS) Stain kit and superoxide dismutase (SOD) kit were purchased from the Nanjing Jiancheng Bioengineering Research Institute Co., Ltd. China. Enzyme-linked immunosorbent assay (ELISA) kit was purchased from the Neobioscience Technology Company (Beijing, China). All reagents used in this study are in analytical grade.
3
Streptozotocin-Induced Diabetic Cell Model
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Streptozotocin (STZ, Sigma-Aldrich, USA); HSYA (mass fraction ≥ 98%; National Institute for the Control of Pharmaceutical and Biological Products, China); foetal bovine serum (FBS) and DMEMF12 medium (Gibco, USA); SuperReal PreMix Kit (Tiangen Biotech (Beijing) Co., Ltd, China); RNAiso Plus Kit, TRIzol Kit and M-PER Kit (Life Technologies, USA); Lipofectamine 2000 Transfection Reagent and Lipofectamine RNAiMAX Kit (Thermo Fisher Scientific, USA); BCA Protein Quantification Kit (Beyotime Biotechnology, Jiangsu, China); miRNA-140-5p mimic and inhibitor (RiboBio, Guangdong, China); rabbit anti-TLR4 polyclonal antibody (Pro-teinTech, USA); rabbit anti-NLRP3 polyclonal antibody (Bioss Antibodies, China); rabbit anti-Notch2 polyclonal antibody (MDL, China); anti-collagen type IV (Col-IV) protein monoclonal antibody (Sigma-Aldrich, USA); anti-β-actin antibody (Boster, Wuhan, China); Total Anti-oxidant Capacity (T-AOC) and Malondialdehyde (MDA) Assay Kits (Nanjing Jiancheng Bioengineering Institute, Nanjing, China); Immunohistochemical Streptavidin-Peroxidase (SP) Two-Step Assay Kit, diaminobenzidine (DAB) substrate solution (ZsBio, Beijing, China); Enzyme-Linked Immunosorbent Assay (ELISA) Kit (NEOBIOSCIENCE, Beijing, China); and Dual-Luciferase Reporter Gene Assay Kit (Promega, USA) were used.
4
Litchi Fruit Polysaccharide Isolation and Characterization
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Fresh litchi fruits (cv. Huai-zhi) were provided by the Fruit Tree Research Institute of Guangdong Academy of Agricultural Sciences (Guangzhou, China). Rhamnose, arabinose, glucose, galactose, mannose, xylose, fucose and standard dextran (T-4: molecular mass, 4.4 × 103 Da, T-10: 9.9 × 103 Da, T-20: 2.14 × 104 Da, T-40: 4.35 × 104 Da, T-100: 1.24 × 105 Da, T-200: 1.96 × 105 Da, T-300: 2.77 × 105 Da, and T-400: 4.01 × 105 Da), lipopolysaccharide (LPS), penicillin and streptomycin were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Dulbecco’s Modified Eagle Medium (DMEM) and fetal bovine serum (FBS) were supplied from Gibco Inc. (Grand Island, NY, USA). Enzyme-linked immunosorbent assay (ELISA) kits were obtained from NeoBioscience (Shenzhen, China). Fructooligosaccharides (DP 2–9) was acquired from Shanghai Ruiyong Biotechnology Co., Ltd. (Shanghai, China). Man-Rogosa-Sharpe (MRS) was supplied from Guangdong Huankai Microbial Technology Co., Ltd. (Guangzhou, China). All other reagents were analytical grade.
5
Synthesis and Characterization of Polymeric Nanomaterials
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1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDCI), triethylamine (TEA), 4-dimethylaminopyridine (DMAP), N,N-diisopropylethylamine (DIEA), N,N-dimethylacetamide (anhydrous, DMAc), dimethyl sulfoxide (anhydrous, DMSO), N,N-dimethylformamide (anhydrous, DMF), dichloromethane (anhydrous, DCM), 2,2′-azobis (2-methylpropionitrile) were purchased from J&K Scientific Ltd. (Shanghai, China). Nile Red (NiR), 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiL) and 1,1′-dioctadecyl-3,3,3′,3′-tetramethyl indotricarbocyanineiodide (DiR) were all obtained from Life Technologies (Shanghai, China). NLG919 was purchased from Selleck Chemghai, China). PPa was purchased from Dibai Chem Tech Co., Ltd. (Shanghai, China). Triphosgene, 4-cyano-4-(dodecylsulfanylthiocarbonyl) sulfanylpentanoic acid (CTA) and bis(2-hydroxyethyl) disulfide were purchased from TCI (Shanghai, China). Enzyme-linked immunosorbent assay (ELISA) kits were ordered from Neobioscience Technology Co., Ltd. (Shenzhen, China). Kyn, Trp and 2,7-dichlorodihydrofluorescein diacetate (DCFH-DA) were ordered from Meilun Biotechnology Co., Ltd. (Dalian, China). The macromolecular chain transfer agent mPEG113-CTA and 2-(hexamethyleneimino) ethanol methacrylate (HMA) were synthenzied by following the similar procedure reported previously34 ,35 (link).
6
Human Hair Extraction and Analysis
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Materials: Human hair was collected from local hair salons in Chongqing, China.
Sodium dodecyl sulfate (SDS), thioglycolic acid (TGA), hydrochloric acid and sodium hydroxide were purchased from Kelong Chemical reagent Co., Ltd. (Chengdu, China).
Amicon ultra-filtration centrifuge tubes (10, 30, 50, and 100 kDa) were purchased from Millipore Corporation (Bedford, USA). Enzyme-linked immunosorbent assay (ELISA) kits were purchased from the Neobioscience Technology Company (Beijing, China). Other reagents used in this study were of analytical grade.
Sodium dodecyl sulfate (SDS), thioglycolic acid (TGA), hydrochloric acid and sodium hydroxide were purchased from Kelong Chemical reagent Co., Ltd. (Chengdu, China).
Amicon ultra-filtration centrifuge tubes (10, 30, 50, and 100 kDa) were purchased from Millipore Corporation (Bedford, USA). Enzyme-linked immunosorbent assay (ELISA) kits were purchased from the Neobioscience Technology Company (Beijing, China). Other reagents used in this study were of analytical grade.
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