Immunoblot analyses were carried out as described previously (13 (link)). Digital images were captured using LAS-1000 Plus (Fujifilm). The following primary antibodies were used: monoclonal anti–Myc 9E10 (Santa Cruz Biotechnology, sc-40), anti–p38α F9 (sc-271120), anti–JNK2 D-2 (sc-7345), anti–ASK1 F-9 (sc-5294); anti–β-actin 6D1 (Wako, 010-27841); anti-Flag M2 (Sigma-Aldrich, F1804); anti–phospho-p38 D3F9 (Cell Signaling Technology, 4511), anti–phospho-JNK 81E11 (4668), anti–phospho-JNK 98F2 (4671), anti–phospho-MAPKAPK2 27B7 (3007), anti–phospho–c-Jun 54B3 (2361); polyclonal anti-Flag (Sigma-Aldrich, F7425); anti-JNK (Cell Signaling Technology, 9252), anti–phospho-MKK3/6 (9231), anti–phospho-MKK4 (9156), anti–phospho-MKK7 (4171), anti-MKK7 (4172), anti-MAPKAPK2 (3042); anti-Trx (Santa Cruz Biotechnology, sc-20146), anti-Myc (sc-789), anti-p38α (sc-535), anti-JNK (sc-571), anti-MKK3/6 (sc-13069), anti-MKK4 (sc-13070), anti-MTK1 (sc-28770), anti–c-jun (sc-1694); and anti-GADD45β (Cloud-Clone, PAL535Hu01). Affinity-purified anti-MTK1 and anti–phospho-MTK1(T1493) antibodies were made in-house (13 (link), 15 (link)). All antibodies were used at a dilution of 1:1000, except anti-ASK1 (1:500) and anti-GADD45β (1:400).
Anti phospho mkk3 6
Manufactured by Santa Cruz Biotechnology
Anti–phospho-MKK3/6 is a primary antibody that specifically binds to the phosphorylated forms of the mitogen-activated protein kinase kinase 3 (MKK3) and mitogen-activated protein kinase kinase 6 (MKK6) proteins. These proteins are important regulators of the p38 MAPK signaling pathway.
Automatically generated - may contain errors
Lab products found in correlation
Anti trx, by Santa Cruz Biotechnology (1 mentions)
Anti phospho p38 d3f9, by Cell Signaling Technology (1 mentions)
Anti β actin 6d1, by Fujifilm (1 mentions)
Las 1000plus, by Fujifilm (1 mentions)
Anti myc 9e10, by Santa Cruz Biotechnology (1 mentions)
Anti jnk, by Cell Signaling Technology (1 mentions)
Anti flag, by Merck Group (1 mentions)
Anti flag m2, by Merck Group (1 mentions)
Anti tubulin, by Cell Signaling Technology (1 mentions)
Anti cleaved caspase 3, by Santa Cruz Biotechnology (1 mentions)
Anti jnk, by Merck Group (1 mentions)
Sb202190, by Bio-Techne (1 mentions)
Anti erk1 2, by Merck Group (1 mentions)
Anti phospho erk1 2, by Merck Group (1 mentions)
Recombinant bfgf, by Merck Group (1 mentions)
Propidium iodide, by Merck Group (1 mentions)
Anti gfp, by Roche (1 mentions)
2 protocols using anti phospho mkk3 6
1
Immunoblot Analysis of Stress Signaling
2
Investigating MAPK Pathway Inhibitors
Check if the same lab product or an alternative is used in the 5 most similar protocols
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MEK1/2 inhibitor U0126 and p38α/β inhibitor SB203580 were purchased from Calbiochem, and JNK1-3 inhibitor SP600125 was from Biomol. Recombinant bFGF was purchased from Sigma. The following primary antibodies were used: anti-MKK3/6, anti-phospho MKK3/6, anti-MKK4, anti-MKK7 from Santa-Cruz Biotechnology, anticleaved caspase-3, anti phospho JNK1-3, anti-phospho MKK4, anti-phospho MKK7, anti-phospho c-Jun, antiphospho MAPKAPK2, anti-pSer473-AKT (pAKT), anti-general-AKT (gAKT), anti-GST and anti-GFP from Cell Signaling, anti-tubulin, anti-ERK1/2, anti-phospho ERK1/2, anti-JNK, ant-p38α, anti-phosphor-p38, anti-c-Jun and anti-MAPKAPK2 from Sigma, and anti-GFP from Roche. Propidium iodide was purchased from Sigma-Aldrich (St Louis, MO, USA). SB202190, RWJ67567, and BI78D3, inhibitors were from Tocris Bioscience (Ellisville, MO, USA). antibodies were from Cell Signaling (Beverly, MA, USA).
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