Split splitless auto injector

For FA profile determination, a transmethylation with KOH in methanol was performed on the extract to obtain methyl esters, according to ISO 12966-2:2017 [128 (link)]. The obtained extract was then injected in a GC-FID system (Shimadzu, Tokyo, Japan) equipped with an AOC-20i automatic sampler and a split/splitless auto injector (Shimadzu, Tokyo, Japan) at 250 °C, a flame ionization detector (Shimadzu, Tokyo, Japan) at 270 °C, and a CP-Sil 88 silica capillary column (50.0 m × 0.25 mm inner diameter and 0.20 μm film thickness, Varian, Middelburg, Netherlands). The carrier gas was helium. The injection volume was 1 μL. The used program was: 120 °C held for 5 min, 2 °C/min to 160 °C held for 2 min, and 2 °C/min to 220 °C held for 10 min. Identification was performed by comparing the retention times of fatty acids methyl esters to a standard mixture (FAME 37, Supelco, Bellefonte, PA, USA). The data were analyzed based on relative peak areas. Results are expressed as a relative percentage (%) of total FA.

For FA profile determination, a transmethylation with KOH in methanol was performed to the extract to obtain methyl esters, according to ISO 12966-2:2017 [43 ]. The obtained extract was then injected in a GC-FID system (Shimadzu, Tokyo, Japan) equipped with an AOC-20i automatic sampler and a split/splitless auto injector (Shimadzu, Tokyo, Japan) at 250 °C, a flame ionization detector (Shimadzu, Tokyo, Japan) at 270 °C, and a CP-Sil 88 silica capillary column (50.0 m × 0.25 mm inner diameter and 0.20 μm film thickness, Varian, Middelburg, Netherlands). The carrier gas was helium. The injection volume was 1 μL. The used program was: 120 °C held for 5 min, 2 °C/min to 160 °C held for 2 min, and 2 °C/min to 220 °C held for 10 min. Identification was performed by comparing the retention times of fatty acids methyl esters to a standard mixture (FAME 37, Supelco, Bellefonte, PA, USA). The data were analyzed based on relative peak areas. Results are expressed as relative percentage (%) of total FA.