Arachidonoyl chloride

1-O-1′-(Z)-hexadecenyl-2-oleoyl-sn-glycero-3-phosphocholine, 1-O-1′-(Z)-octadecenyl-2-arachidonoyl-sn-glycero-3-phosphocholine, 1-palmitoyl-2-oleoyl-sn-glycerol and 1-stearoyl-2-arachidonoyl-sn-glycerol were purchased from Avanti Polar Lipids. 1-O-hexadecyl-sn-glycero-3-phosphocholine and 1-O-octadecyl-sn-glycero-3-phosphocholine were obtained from Cayman Chemical. Arachidonoyl chloride was purchased from Santa Cruz Biotechnology. Oleoyl chloride, N,N-dimethylglycine (DMG) hydrochloride, 4-(dimethylamino)pyridine, and Bacillus cereus phospholipase C (PLC) were obtained from Sigma Aldrich. Triethylamine was purchased from Fisher Scientific. Solvents for mass spectrometric analysis were purchased from Burdick and Jackson (Muskegon, MI).

Resorufin, Resorufin sodium salt, naphthofluorescein, palmitoyl chloride, phospholipase A₂ from porcine pancreas, arachidonyl trifluoromethyl ketone, bromoenol lactone, ionomycin, and H₂O₂ 30% (w/w) were purchased from Sigma-Aldrich. DDAO, arachidonoyl chloride, and LY311727 were purchased from Santa Cruz Biotechnology. 7-HCA was purchased from Cayman Chemical. Syto 9 was purchased from Life Technologies. Egg phosphatidylcholine was purchased from Avanti Polar Lipids, Inc.