hdcA43 was generated by the EMS mutagenesis. hdcdel was generated by the FRT-FLP-mediated genomic deletion strategy using two FRT-containing Exelexis lines (d05275 and e03709) according to standard procedures. A full-length hdc cDNA (LD44381) was used to make UAS-hdc transgenic flies. A full-length unk cDNA (RE58038) was used to make UAS-unk transgenic flies. raptordel was generated by homologous recombination strategy (Gong and Golic, 2003 (link); Zhang, 2009 ). unkex24 (Avet-Rochex et al., 2014 (link)), tsc129 (Gao and Pan, 2001 (link)), and Tor2L1 (Oldham et al., 2000 (link)) mutant flies were previously reported. UAS-p35 was obtained from Bloomington Stock Center (stock number: 5073). All RNAi flies were obtained from Bloomington Stock Center (UAS-unkRNAi: 57026, UAS-hdcRNAi: 30489, UAS-TorRNAi: 35578, UAS-raptorRNAi: 31529).
Double mutant clones in the eye imaginal discs were generated using flies containing double FRT chromosomes with GFP and RFP markers together with an eye-specific FLP source as described before (Yu and Pan, 2018 (link)).
Double mutant clones in the eye imaginal discs were generated using flies containing double FRT chromosomes with GFP and RFP markers together with an eye-specific FLP source as described before (Yu and Pan, 2018 (link)).