Phospho ulk s757

The following antibodies were purchased from Cell Signaling and used at the indicated dilution for Western blot analysis: S6K1 (#9202, 1 : 1000), phospho-S6K1 (#9234, 1 : 1000), AKT (#9272S, 1 : 1000), phospho-AKT S437 (#4058S, 1 : 1000), 4EBP1 (#9452, 1 : 1500), phospho-4EBP1 (#9451, 1 : 1000) ULK (8054S, 1 : 1000), phospho-ULK S757 (#6888, 1 : 1000), FoxO3a (12829S, 1 : 1000), phospho-FoxO1(T24)/FoxO3a(T32) (9464T, 1 : 1000), GSK3β (12456S, 1 : 1000), phospho-GSK3α/β S21/9 (12456S, 1 : 1000), TSC2 (3635S, 1 : 1000), phospho-TSC2 (3617S, 1 : 1000), mTOR (#2983, 1 : 1000), Raptor (#2280, 1 : 1000), mlST8 (#3274, 1 : 1000), DEPTOR (#11816, 1 : 1000), PRAS40 (#2691, 1 : 1000), Sin1 (#12860S, 1 : 1000), phospho-CREB (#9198S, 1 : 1000), CREB (#9197S, 1 : 1000), phospho-Thr (#9381S, 1 : 1000), phospho-β-catenin (#9561, 1 : 1000), PKA Cat α (#4782, 1 : 1000), phospho-glycogen synthase (#3891, 1 : 1000), and actin (#3700, 1 : 1000). Flag (#F3165, 1 : 10 000) was obtained from Sigma. HA (#sc-7392 or #sc-805, 1 : 500) and Myc (#sc-40, 1 : 1000), FKBP12 (#sc-133067, 1 : 1000) were obtained from Santa Cruz. EGFP (#632380, 1 : 2000) was from Clontech. Horseradish peroxidase (HRP) linked secondary antibodies (#NXA931V anti-mouse, 1 : 8000 or #NA934V anti-rabbit, 1 : 4000) were from GE Healthcare.

Antibodies used in this study were as follows: ATG16L1 (8089, human), phospho-ATG14 S29 (92340), ATG14 (96752), phospho-Beclin S30 (54101), FIP200 (12436), FLCN (3697), GABARAPL1 (26632), GABARAPL2 (14256), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) [5174, 1:10,000 for WB (Western blot)], DYKDDDDK tag (14793), HA tag (3724), myc tag (2278), LC3A/B (12741), LC3B (3868), LAMTOR1 (8975), LAMP1 [15665, 1:1000 for immunofluorescence (IF)], NFAT1 (5861, 1:250 for IF), NPRL2 (37344), phospho-S6K (9234), S6K (2708), phospho-S6 S235/236 (4858, 1:3000 for WB), S6 (2217, 1:5000 for WB), TAX1BP1 (5105), TFEB (4240), TFEB (37785, 1:200 for IF), and phospho-ULK S757 (14202) were from Cell Signaling Technology. Mouse monoclonal anti–S. Typhimurium lipopolysaccharide (clone 1E6, ab8274) and FNIP1 (ab134969) were from Abcam. TFE3 (HPA023881) was from MilliporeSigma. p62 (GP62-C) was from Progen. Galectin-3 (sc-23938) was from Santa Cruz Biotechnology. TFEB (A303-673A, 1:200 for IF in murine cells) was from Bethyl Laboratories. All antibodies were used at a 1:1000 dilution for Western blotting unless otherwise noted.