Ltq orbitrap elite ftms

Mass spectra were recorded on an LTQ Orbitrap Elite FTMS instrument (LTQ Orbitrap Elite FTMS, Thermo Scientific, Bremen, Germany). For operation in negative mode, the orbitrap was interfaced with a HESI-II probe in an Ion Max ion Source. The ionization voltage was set at  − 1.2 kV and the ion transfer capillary temperature at 120 °C. For operation in positive ion mode, the orbitrap was interfaced with a robotic chip-based nano-ESI source (TriVersa Nanomate, Advion Biosciences, Ithaca, NY, USA). Standard data acquisition and instrument control system were used (Thermo Scientific), and the ion source was controlled by the Chipsoft 8.3.1 software (Advion BioScience). Samples were loaded onto a 96-well plate (Eppendorf, Hamburg, Germany) with an injection volume of 5 µl. The ionization voltage was set at + 1.4 kV, the gas pressure at 0.30 psi and the temperature of ion transfer capillary at 200 °C. For tandem MS data analysis, platinum-containing adducts were fragmented by collision-induced dissociation (CID) in the linear ion trap using an isolation window of 8 Da, with product ion detected in the Orbitrap with a resolution set to 120 K.