Cfi apo tirf 100x 1.49 n a oil objective

Manufactured by Nikon

The CFI Apo TIRF 100X 1.49 N.A. oil objective is a high-performance microscope objective designed for Total Internal Reflection Fluorescence (TIRF) microscopy. It features a 100X magnification and a numerical aperture of 1.49, which enables high-resolution imaging of samples near the coverslip surface. The objective is optimized for use with oil immersion to provide superior optical performance.

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Lab products found in correlation

Eclipse ti e, by Nikon (2 mentions) Prism 6, by GraphPad (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) N sim, by Nikon (1 mentions) A1 laser scanning confocal microscope, by Nikon (1 mentions) Photometrics evolve 512 emccd, by Teledyne (1 mentions) Metamorph 7, by Molecular Devices (1 mentions) Evolve 512 emccd camera, by Teledyne (1 mentions) Optosplit 3 beamsplitter, by Cairn Research (1 mentions)

Spelling variants of this product

Apo TIRF objective (13 citations) CFI Apo TIRF (10 citations) CFI Apo TIRF 1.49 N.A. oil objective (3 citations) Apo TIRF 100x/1.49 oil objective (3 citations) CFI Apo TIRF 100x (3 citations) Apo TIRF N.A. 1.49 (3 citations) CFI APO TIRF 100x/1.49 oil objective (2 citations) CFI Apo TIRF 100X oil objective (2 citations) Apo TIRF 100x 1.49 objective (2 citations) 60 × 1.49 N.A. oil objective (2 citations)

4 protocols using cfi apo tirf 100x 1.49 n a oil objective

Imaging EB1-GFP dynamics in ATIP3-depleted HeLa cells

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HeLa cells were transfected for 24 h with 20 nM control siRNA (siCtrl) or ATIP3-specific-siRNA (siATIP3) using Lipofectamine 2000 (Invitrogen), then transfected for 24 h with 4 µg EB1-GFP construct as described^{17 (link)}. Transfected cells were treated with DMSO or paclitaxel (5 nM) during 2h30 before being imaged on a Nikon Eclipse Ti microscope with the PFS (Perfect Focus System), equipped with a Nikon CFI Apo TIRF 100X 1.49 N.A. oil objective and controlled with MetaMorph software. Images were acquired in a stream mode at 25 ms exposure time during 30 s. Image analysis was performed with ImageJ software, version 1.51 k (W. Rasband, NIH, USA) and statistical analyses were done with Prism 6.0 (GraphPad software, USA).

Rodrigues-Ferreira S., Nehlig A., Kacem M, & Nahmias C. (2020). ATIP3 deficiency facilitates intracellular accumulation of paclitaxel to reduce cancer cell migration and lymph node metastasis in breast cancer patients. Scientific Reports, 10, 13217.

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High-Resolution Confocal and Super-Resolution Microscopy

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Confocal microscopy was performed using a Nikon A1 laser scanning confocal microscope. In Figures 4A and S4, to enhance spatial resolution, we used the Nikon enhanced resolution (ER) module (Nikon A1 ER using a 60X 1.4NA oil objective). Raw data for ER were processed using the Richardson-Lucy deconvolution algorithm of Nikon Elements software. Super resolution microscopy (3D-SIM) was performed using a Nikon N-SIM. All data for 3D-SIM were captured used a Nikon CFI Apo TIRF 100X 1.49NA oil objective. 3D-SIM image stacks were sectioned using a 200 nm Z-step size. Raw data for 3D-SIM were reconstructed and analyzed using Nikon Elements software.

Devergne O., Sun G.H, & Schüpbach T. (2017). Stratum, a Homolog of the Human GEF Mss4, Partnered with Rab8, Controls the Basal Restriction of Basement Membrane Proteins in Polarized Epithelial Cells. Cell reports, 18(8), 1831-1839.

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Microtubule Dynamics in EGFR Mutants

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MaMTH reporter HEK293 cells stably expressing EGFR WT or EGFR L858R/T790M/C797S were transfected with EB3-TagRFP. Cell were stimulated with 0.5 µg/ml tetracycline to induce EGFR expression 18 hours prior to imaging. Cells were incubated with EMI for 30 min prior to imaging. Inverted research microscope Nikon Eclipse Ti-E (Nikon) supplemented with the perfect focus system (PFS) (Nikon) and equipped with Nikon CFI Apo TIRF 100x 1.49 N.A. oil objective (Nikon), Photometrics Evolve 512 EMCCD (Roper Scientific) controlled with MetaMorph 7.7 software (Molecular Devices) was used to perform the live cell imaging. Images were acquired in a stream mode with an exposure time of 500 ms. Kymographs were generated using ImageJ plugin KymoResliceWide (https://github.com/ekatrukha/KymoResliceWide). Parameters of microtubule dynamics were analyzed as described previously^{41 ,42}.

Saraon P., Snider J., Kalaidzidis Y., Wybenga-Groot L.E., Weiss K., Rai A., Radulovich N., Drecun L., Vučković N., Vučetić A., Wong V., Thériault B., Pham N.A., Park J.H., Datti A., Wang J., Pathmanathan S., Aboualizadeh F., Lyakisheva A., Yao Z., Wang Y., Joseph B., Aman A., Moran M.F., Prakesch M., Poda G., Marcellus R., Uehling D., Samaržija M., Jakopović M., Tsao M.S., Shepherd F.A., Sacher A., Leighl N., Akhmanova A., Al-awar R., Zerial M, & Stagljar I. (2020). A drug discovery platform to identify compounds that inhibit EGFR triple mutants. Nature chemical biology, 16(5), 577-586.

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Real-time Microtubule Dynamics Visualization

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Reconstitution assay was performed as previously described (Doodhi et al., 2016) . The reaction mixtures in MRB80 buffer contained tubulin (15 mM), Rhodamine-tubulin (0.5 mM) when indicated, methyl cellulose (0.1%), KCl (50 mM), k-casein (0.5 mg/ml), GTP (1 mM), oxygen scavenging system (20 mM glucose, 200 mg/ml catalase, 400 mg/ml glucose-oxidase, 4 mM DTT), mCherry-EB3 or mCherry-EB3Dtail (20 nM each) and Kar9c-GFP (0.64 mM). Movies were acquired in TIRF mode using Nikon Eclipse Ti-E (Nikon) microscope supplemented with the perfect focus system (PFS) (Nikon), equipped with Nikon CFI Apo TIRF 100x 1.49 N.A. oil objective (Nikon). Photometrics Evolve 512 EMCCD camera (Roper Scientific) with triple-band TIRF polychroic ZT405/488/561rpc (Chroma) and tripleband laser emission filter ZET405/488/561m (Chroma), mounted in the metal cube (Chroma, 91032) together with Optosplit III beamsplitter (Cairn Research Ltd, UK) supplemented with double emission filter cube configured with ET525/50m, ET630/75m and T585LPXR (Chroma) was used to acquire the movies in stream acquisition mode (exposure time 500 ms). Kymographs were generated in ImageJ using KymoResliceWide plugin.

Kumar A., Manatschal C., Rai A., Grigoriev I., Degen M.S., Jaussi R., Kretzschmar I., Prota A.E., Volkmer R., Kammerer R.A., Akhmanova A, & Steinmetz M.O. (2017). Short Linear Sequence Motif LxxPTPh Targets Diverse Proteins to Growing Microtubule Ends. Structure (London, England : 1993), 25(6).

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