4 nitrophenyl octanoate

Solvents of analytical grade were obtained from VWR International s.r.l. (Milan, Italy).
Tween 20, ascorbic acid, Folin–Ciocalteu reagent, sodium carbonate, butylated hydroxytoluene (BHT), propyl gallate, 2,2-diphenyl-1-picrylhydrazyl (DPPH), tripyridyltriazine (TPTZ), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic) acid (ABTS) solution, β-carotene, linoleic acid, Orlistat, Trizma base, 4-nitrophenyl octanoate (NPC), maltose, α-amylase from porcine pancreas, α-glucosidase from Saccharomyces cerevisiae, o-dianisidine dihydrochloride, and peroxidase/glucose oxidase (PGO) were purchased from Sigma–Aldrich S.p.a. (Milan, Italy).
Acarbose from Actinoplanes sp. was obtained from Serva (Heidelberg, Germany). Caffeic acid, protocactechuic acid, p-coumaric acid, chlorogenic acid, vanillic acid, eriocitrin, gallic acid, apigenin, didymin, quercetin, hesperidin, neohesperidin, neoeriocitrin, naringin, narirutin, sinensetin, tangeretin, rutin, quercetin-O-glucoside, genistin, poncirin, luteolin, kaempferol, hesperetin, rhamnetin, umbelliferone, isopimpinellin, and bergapten were purchased from Sigma–Aldrich Chem. Co. (Milwaukee, WI, USA). Acetonitrile, formic acid, methanol, and water were HPLC-grade solvents, obtained from Carlo Erba Reagents (Milano, Italia).

Folin–Ciocalteu reagent, sodium carbonate, rutin standard, sodium nitrite, aluminum trichloride, methanol, gallic acid, orlistat, type II crude porcine pancreatic lipase, 4-nitrophenyl octanoate (NPC), dimethylsulfoxide, Tris-HCl buffer (pH = 8.5), acarbose, potato starch, sodium phosphate buffer, sodium chloride, α-amylase, sodium potassium tartrate tetrahydrate, sodium hydroxide, and 3,5-dinitro salicylic acid were all purchased from Sigma Aldrich, Munich, Germany. A FluoStar Omega microplate reader (Shimadzu UV-1650PC, Kyoto, Japan) was used for the determination of total phenolics and flavonoid content. A plate reader (Model: BioTek ELX 808, Milano, Italy) was also used for a pancreatic lipase inhibition test. MaoFeng green tea (cultivated in China) and Bai Mu Dan white tea (cultivated in China) were purchased from the United Arab Emirates, while Tieguanyin Oolong tea (cultivated in Anxi Fujian in China) was purchased from Malaysia. Ethanol (95% (v/v)) was obtained from Sigma Aldrich (Germany).