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Rat igg2a κ isotype control clone rtk2758

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The Rat IgG2a-κ isotype control (clone RTK2758) is a laboratory reagent used in flow cytometry and other immunoassays to establish appropriate background staining levels. This isotype control antibody shares the same isotype (IgG2a) and light chain (κ) as many rat monoclonal antibodies, but it does not bind to any known antigen.

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Lab products found in correlation

Annexin 5, by BioLegend (2 mentions) Anti β actin clone c4, by Santa Cruz Biotechnology (2 mentions) Caspace fitc vad fmk probe for active caspases, by Promega (2 mentions) Anti ps6 clone d57.2.2e, by Cell Signaling Technology (2 mentions) Anti vdac1 clone 20b12af2, by Abcam (2 mentions) Anti coxiv clone 20e8c12, by Abcam (2 mentions) Polyclonal anti calnexin catalogue a303 696a, by Fortis Life Sciences (2 mentions) Polyclonal anti stat5 catalog number c 17, by Santa Cruz Biotechnology (2 mentions) Anti ubiquitin antibody clone fk2, by Enzo Life Sciences (2 mentions) Blocking il7rα clone a7r34 32 link, by BioXCell (2 mentions) Propidium iodide, by BioLegend (2 mentions) Anti s1pr1 clone 713412, by R&D Systems (2 mentions) Isotype control clone 2a3, by BioXCell (2 mentions)

Spelling variants of this product

Isotype control (56 citations) Rat IgG2a (27 citations) IgG2a (24 citations) RTK2758 (18 citations) IgG2a isotype control (8 citations) Clone RTK2758 (8 citations) Rat IgG2a clone RTK2758 (2 citations) Rat IgG2a (RTK2758, (2 citations)

2 protocols using rat igg2a κ isotype control clone rtk2758

1

Comprehensive Immune Cell Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies to B220 (clone RA3-6B2), CD4 (clone RMA4-5 or GK1.5), CD8 (clone 53-6.7), CD62L (clone MEL14), CD69 (clone H1.2F3), IL7Rα (clone A7R34), CD44 (clone IM7), Nur77 (clone 12.14), Lyve1 (clone ALY7), MHCII (clone M5/114.12.2), gp38 (clone 8.1.1), CD25 (clone PC61), PINK1 (clone 5E1.D8), and rat IgG2a-κ isotype control (clone RTK2758) were purchased from Biolegend, eBiosciences, or BD Biosciences. Anti-S1PR1 (clone 713412) was purchased from R&D Systems. Anti-pSTAT5 (clone D47E7), anti-AKT (catalogue # 9272), anti-pAKT (clone D9E), anti-S6 (clone 5G10) and anti-pS6 (clone D57.2.2E) were purchased from Cell Signaling Technologies. Anti-VDAC1 (clone 20B12AF2) and anti-COXIV (clone 20E8C12) were purchased from Abcam. Polyclonal anti-calnexin (catalogue #A303-696A) was purchased from Bethyl Laboratories. Polyclonal anti-STAT5 (catalog number C-17) and anti-β–actin (clone C4) were purchased from Santa Cruz Biotechnology. Anti-ubiquitin antibody (clone FK2) was purchased from Enzo Life Sciences. Blocking IL7Rα (clone A7R34)32 (link) and isotype control (clone 2A3) antibodies were purchased from Bioxcell. AnnexinV and propidium iodide were purchased from Biolegend. CaspACE FITC-VAD-FMK probe for active caspases was purchased from Promega. For flow cytometry, cells were analyzed using an LSRII (BD Biosciences); data were analyzed using FlowJo v. 8.8.1 (Tree Star).
Mendoza A., Fang V., Chen C., Serasinghe M., Verma A., Muller J., Chaluvadi V.S., Dustin M.L., Hla T., Elemento O., Chipuk J.E, & Schwab S.R. (2017). Lymphatic endothelial S1P promotes naïve T cell mitochondrial function and survival. Nature, 546(7656), 158-161.
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2

Comprehensive Immune Cell Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies to B220 (clone RA3-6B2), CD4 (clone RMA4-5 or GK1.5), CD8 (clone 53-6.7), CD62L (clone MEL14), CD69 (clone H1.2F3), IL7Rα (clone A7R34), CD44 (clone IM7), Nur77 (clone 12.14), Lyve1 (clone ALY7), MHCII (clone M5/114.12.2), gp38 (clone 8.1.1), CD25 (clone PC61), PINK1 (clone 5E1.D8), and rat IgG2a-κ isotype control (clone RTK2758) were purchased from Biolegend, eBiosciences, or BD Biosciences. Anti-S1PR1 (clone 713412) was purchased from R&D Systems. Anti-pSTAT5 (clone D47E7), anti-AKT (catalogue # 9272), anti-pAKT (clone D9E), anti-S6 (clone 5G10) and anti-pS6 (clone D57.2.2E) were purchased from Cell Signaling Technologies. Anti-VDAC1 (clone 20B12AF2) and anti-COXIV (clone 20E8C12) were purchased from Abcam. Polyclonal anti-calnexin (catalogue #A303-696A) was purchased from Bethyl Laboratories. Polyclonal anti-STAT5 (catalog number C-17) and anti-β–actin (clone C4) were purchased from Santa Cruz Biotechnology. Anti-ubiquitin antibody (clone FK2) was purchased from Enzo Life Sciences. Blocking IL7Rα (clone A7R34)32 (link) and isotype control (clone 2A3) antibodies were purchased from Bioxcell. AnnexinV and propidium iodide were purchased from Biolegend. CaspACE FITC-VAD-FMK probe for active caspases was purchased from Promega. For flow cytometry, cells were analyzed using an LSRII (BD Biosciences); data were analyzed using FlowJo v. 8.8.1 (Tree Star).
Mendoza A., Fang V., Chen C., Serasinghe M., Verma A., Muller J., Chaluvadi V.S., Dustin M.L., Hla T., Elemento O., Chipuk J.E, & Schwab S.R. (2017). Lymphatic endothelial S1P promotes naïve T cell mitochondrial function and survival. Nature, 546(7656), 158-161.
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