Total RNA was Trizol-extracted, column-purified and reverse-transcribed using PrimeScript 1st Strand Cdna Synthesis kit (Takara). Primer sequences used to test gene expression are available in Supplementary Table 1 . ChIP was performed according to the manufacturer's instructions (CST, #9004). For ChIP–qPCR analysis, 1 ng ChIP DNA was used for each PCR. All qPCR analyses were performed using Fast SYBR Green (Takara). Primer sequences are available in Supplementary Table 2 .
Fast sybr green
Manufactured by Takara Bio
Fast SYBR Green is a real-time PCR reagent designed for rapid and sensitive detection of target DNA sequences. It utilizes the SYBR Green dye, which binds to double-stranded DNA, enabling quantification of the target DNA. The reagent is formulated for fast thermal cycling protocols, allowing for efficient and time-saving DNA amplification and detection.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using fast sybr green
1
RNA Extraction, cDNA Synthesis, and ChIP-qPCR Analysis
2
Total RNA Extraction and cDNA Synthesis
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Total RNA was Trizol-extracted, column-purified and reverse-transcribed using PrimeScript 1st Strand Cdna Synthesis kit (Takara). All qPCR analyses were performed using Fast SYBR Green (Takara).
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