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Cxcr2 242216

Manufactured by Thermo Fisher Scientific

CXCR2 (242216) is a lab equipment product from Thermo Fisher Scientific. It is a receptor protein that plays a role in cellular signaling processes. The core function of this product is to facilitate the study of CXCR2 and its related biological mechanisms.

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2 protocols using cxcr2 242216

1

Imaging Neutrophil Migration in Vitro

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Function blocking CD11b (M1/70) and CXCR2 (242216) mAbs from eBioscience (San Diego, CA). An anti-Ly-6G and anti-CD11b conjugated to Alexa Fluor® 488 from Tonbo Bosciences (San Diego, CA). Non-blocking anti-PECAM- 1 antibody (clone 390) was purchased from EMD Millipore (Burlington, MA) and conjugated to DyLight®−550 using an antibody labeling kit from Thermo Fisher (Waltham, MA) according to the manufacture’s instructions. PMN chemoattractants CXCL1 (KC) and CXCL2 (MIP2) from Peprotec (Rocky Hill, NJ). Rat monoclonal E- cadherin (DECMA-1) from ABCAM (Cambridge, MA). Phalloidin conjugated to Alexa Fluor® 647 from Invitrogen (Carlsbad, CA). Texas Red-labeled dextran (70 kDa) from Molecular Probes (Eugene, OR). Lipopolysaccharide (LPS), HBSS with Ca2+ and Mg2+ (HBSS+) and HBSS without Ca2+ and Mg2+ (HBSS-) from Sigma (St Louis, MO).
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2

Imaging Neutrophil Migration in Vitro

Check if the same lab product or an alternative is used in the 5 most similar protocols
Function blocking CD11b (M1/70) and CXCR2 (242216) mAbs from eBioscience (San Diego, CA). An anti-Ly-6G and anti-CD11b conjugated to Alexa Fluor® 488 from Tonbo Bosciences (San Diego, CA). Non-blocking anti-PECAM- 1 antibody (clone 390) was purchased from EMD Millipore (Burlington, MA) and conjugated to DyLight®−550 using an antibody labeling kit from Thermo Fisher (Waltham, MA) according to the manufacture’s instructions. PMN chemoattractants CXCL1 (KC) and CXCL2 (MIP2) from Peprotec (Rocky Hill, NJ). Rat monoclonal E- cadherin (DECMA-1) from ABCAM (Cambridge, MA). Phalloidin conjugated to Alexa Fluor® 647 from Invitrogen (Carlsbad, CA). Texas Red-labeled dextran (70 kDa) from Molecular Probes (Eugene, OR). Lipopolysaccharide (LPS), HBSS with Ca2+ and Mg2+ (HBSS+) and HBSS without Ca2+ and Mg2+ (HBSS-) from Sigma (St Louis, MO).
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