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Mitotracker cmx ros red

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Mitotracker® CMX-ROS Red is a fluorescent dye that selectively labels mitochondria in live cells. It measures the mitochondrial membrane potential, which is an indicator of mitochondrial function.

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MitoTracker Red (756 citations) MitoTracker (469 citations) Mitotracker CMX-Red (8 citations)

2 protocols using mitotracker cmx ros red

1

Mitochondrial Labeling and Cell Morphology

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The mitochondria were labelled with Mitotracker® CMX-ROS Red (Molecular Probes, Invitrogen, Belgium), which specifically accumulates in the mitochondria based on its membrane potential. To allow evaluation of cell morphology the HCS CellMask™ Blue probe (Molecular Probes, Invitrogen, Belgium) was applied. Again, cells were labeled following 24 h of IONP exposure as explained in Additional file 1.
Joris F., Valdepérez D., Pelaz B., Soenen S.J., Manshian B.B., Parak W.J., De Smedt S.C, & Raemdonck K. (2016). The impact of species and cell type on the nanosafety profile of iron oxide nanoparticles in neural cells. Journal of Nanobiotechnology, 14(1), 69.
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2

Mitochondrial and Cytoplasmic Imaging of IONP Effects

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Cells were seeded at appropriate densities as described in the previous paragraph. The cells were allowed to settle overnight and were subsequently exposed to 3.5, 7, 14, 35 and 70 nM IONP dispersions. After a 24-hour incubation period, the NP dispersions were discarded and the mitochondria and cell cytoplasm were respectively labelled with Mitotracker ® CMX-ROS Red and HCS CellMask ™ Blue (both Molecular Probes, Belgium). Data were obtained with the IN Cell Analyzer 2000 and analysed with the IN Cell Developer Toolbox software. Detailed information on the staining procedure, data acquisition and data analysis is provided in the Supplementary Information.
Joris F., Valdepérez D., Pelaz B., Wang T., Doak S.H., Manshian B.B., Soenen S.J., Parak W.J., De Smedt S.C, & Raemdonck K. (2017). Choose your cell model wisely: The in vitro nanoneurotoxicity of differentially coated iron oxide nanoparticles for neural cell labeling. Acta biomaterialia, 55.
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