Sc 16240

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Sc-16240 is a laboratory equipment product offered by Santa Cruz Biotechnology. It is a specialized device designed for conducting various scientific experiments and analyses. The core function of this product is to facilitate the handling and processing of samples in a controlled and precise manner, as required by researchers and scientists in their work.

Automatically generated - may contain errors

Lab products found in correlation

4 protocols using sc 16240

Immunoblotting for TLR4 and Actin

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immunoblotting was performed as previously reported.^{7 (link)} Membranes were incubated with antibodies against TLR4 (1 : 100; sc-16240; Santa Cruz Biotechnology, Santa Cruz, CA, USA) and actin (1 : 1000; 612656; BD Biosciences).

Semba K., Namekata K., Guo X., Harada C., Harada T, & Mitamura Y. (2014). Renin–angiotensin system regulates neurodegeneration in a mouse model of normal tension glaucoma. Cell Death & Disease, 5(7), e1333-.

+ Open protocol

+ Expand

Antibody Validation for Protein Analysis

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

All procedures were adhered to our previous publication (12 (link)). The following primary antibodies were used: anti-p300 (1:500, sc584, Santa Cruz), anti-C/EBPβ (1:1000, MA1–827, Thermo Fisher), anti-pT188-C/EBPβ (1:1000, 3084, Cell Signaling Technology), anti-TLR4 (1:500, sc16240, Santa Cruz), anti-p38α MAPK (1:500, sc271120, Santa Cruz), anti-p38β MAPK (1:500, 2339, Cell Signaling Technology), anti-p38 MAPK (1:1000, 9212, Cell Signaling Technology), anti-p-p38 MAPK (1:1000, 4511, Cell Signaling Technology), anti-MAFbx (1:1000, AP2041, ECM Bioscience), anti-UBR2 (1:500, NBP1–45243, Novus Biologicals), anti-LC3 (1:2000, NB100–2220, Novus Biologicals) and anti-MHC (1:1000, MAB4470, R&D Systems). Antibody against acetylated Lys-39 of C/EBPβ (1:2000) was generated as previously described (12 (link)). Antibody against phosphorylated Ser-12 of p300 (1:1500) were generated by Pocono Rabbit Farm & Laboratory (Canadensis, PA) from rabbit using the peptide PGPPS(P)AKRPKLSSPAC. The specificity of the antibody was validated using mutated p300 with point mutantions (Figure 1A). Data were normalized to α-Tubulin (Development Studies Hybridoma Bank at the University of Iowa, Iowa City, IA).

Sin T.K., Zhang G., Zhang Z., Zhu J.Z., Zuo Y., Frost J.A., Li M, & Li Y.P. (2020). Cancer-induced muscle wasting requires p38β MAPK-mediated activation of p300. Cancer research, 81(4), 885-897.

+ Open protocol

+ Expand

Comprehensive Antibody Immunodetection Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

A goat polyclonal antibody against TLR4 (sc-16,240), a rabbit polyclonal IgG antibody (sc-28,999) against TLR3 (M-300), and a rabbit polyclonal IgG antibody (sc-30,004) against TLR7 (H-114) were purchased from Santa Cruz Biotechnology (TX, USA). A rabbit polyclonal antibody against C23 (ab22758), a mouse monoclonal antibody against F protein (RSV3216 (B016), ab24011), an anti-NeuN antibody (EPR12763, neuronal marker, ab177487), a donkey anti-goat IgG heavy and light chain (H&L) antibody (Alexa Fluor 555, ab150130) against TLR4, a goat anti-rabbit IgG H&L antibody (Alexa Fluor 488, ab150077) against C23, and a goat anti-mouse IgG H&L antibody (Alexa Fluor 647, ab150115) against RSV F were purchased from Abcam (Cambridge, UK). The Annexin V-FITC apoptosis kit (BB-4101) was obtained from BestBio (Shanghai, China), and enzyme-linked immunosorbent assay (ELISA) kits for IL-6 and TNF-α were obtained from Hermes Criterion Biotechnology (Vancouver, Canada).

Yuan X., Hu T., He H., Qiu H., Wu X., Chen J., Wang M., Chen C, & Huang S. (2018). Respiratory syncytial virus prolifically infects N2a neuronal cells, leading to TLR4 and nucleolin protein modulations and RSV F protein co-localization with TLR4 and nucleolin. Journal of Biomedical Science, 25, 13.

+ Open protocol

+ Expand

Immunohistochemical Analysis of Neural Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Paraffin-embedded sections (7-mm thick) were examined using the following primary antibodies: mouse anti-NF200
(1:400; Sigma), mouse antieglial fibrillary acidic protein (GFAP) (50 mg/mL; Progen, Heidelberg, Germany), goat anti-iba1 (1:400; Abcam, Cambridge, England), goat anti-TLR4 (2 mg/mL; sc-16240; Santa Cruz Biotechnology, Santa Cruz, CA), and rabbit antieNF-kB p65 (1:500; catalog number 8242; Cell Signaling, Danvers, MA). The antibodies against TLR-4 18,24,30,31 and NF-kB 32, 33 have been widely used and described elsewhere. Stained sections were examined using a microscope (BX51; Olympus Corporation, Tokyo, Japan) connected to a DP70 camera (Olympus). Images were processed and viewed using DP manager software version 2.2. 1.195 (Olympus). Quantitative analysis of the stained region was performed using ImageJ software version 1.46r (NIH, Bethesda, MD; http://imagej.nih.gov/ij).

Guo X., Namekata K., Kimura A., Harada C, & Harada T. (2017). The Renin-Angiotensin System Regulates Neurodegeneration in a Mouse Model of Optic Neuritis. The American journal of pathology, 187(12).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!