Autopsied lung tissue (FFPE) was freshly cut into 5 μm sections, protease 3 (Ventana, 760-2020) treated, heated to induce epitope retrieval with alkaline CC1 buffer (Ventana, 950-124), and stained on a Ventana Benchmark Ultra (Ventana Medical Systems, Tucson, AZ, USA) with AE1/AE3 (Ventana, 760-2135). Parallel sections were stained with H&E and AE1/AE3 to distinguish sloughed pneumocytes from immune cells. Visualization with DAB was performed via Optiview detection (Ventana, 760-700). All tissues from patients with COVID-19 were collected post-mortem at the time of autopsy at Boston Medical Center with consent from next-of-kin, and the IRB of Boston University determined that this study did not constitute human subject research. Control samples without infection were collected from healthy tissue adjacent to a lung tumor, with IRB approval under protocol H-37859.
Ae1 ae3
Manufactured by Roche
Sourced in United States
The AE1/AE3 is a monoclonal antibody that binds to different cytokeratin subtypes, allowing for the identification of cells of epithelial origin in immunohistochemical applications.
Automatically generated - may contain errors
Lab products found in correlation
Ventana benchmark ultra, by Roche (1 mentions)
Protease 3, by Roche (1 mentions)
Envision flex hrp polymer, by Agilent Technologies (1 mentions)
Foxp3 236a e7, by Abcam (1 mentions)
Cd8 c8 144b, by Agilent Technologies (1 mentions)
Cd68 pgm 1, by Agilent Technologies (1 mentions)
Cd31 jc70a, by Agilent Technologies (1 mentions)
3 3 diaminobenzidine (dab), by Agilent Technologies (1 mentions)
Autostainer link 48, by Agilent Technologies (1 mentions)
2 protocols using ae1 ae3
1
Immunohistochemical Analysis of COVID-19 Lung Tissue
2
Evaluating PD-L1 Expression in HCC Samples
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To determine PD-L1 expression in HCC resection samples, serial 4–5 μm sections were stained with hematoxylin and eosin for histopathological assessment and were used for IHC. The diagnosis of HCC and the histologic subtype were confirmed independently by two board-certified pathologists (LMT and CI). Histologic grading and classification of HCCs were performed according to the World Health Organization (WHO) guidelines and the Edmondson & Steiner grading system (22 ). IHC was carried out using an Autostainer link 48 (Dako) with primary monoclonal antibodies recognizing PD-L1 (MKP-1A-73-10; PharmDx), CD8 (C8/144B; Dako), CD31 (JC70A; Dako), CD68 (PG-M1; Dako), FoxP3 (236A/E7; Abcam), and pan cytokeratin (AE1/AE3 Ventana). Envision FLEX HRP polymer and DAB+ (Dako) secondary reagents were used. For double labeling, the LabVision™ Multivision Polymer Detection System (ThermoScientific) was used according to the manufacturer's directions.
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