The removed femur and tibiae bones were fixed in 10% neutral buffered formalin. Before CT scanning, they were washed with running water for 15 min. The imaging of 3D microcomputed tomography was performed with a CompactCT micro‐CT scanner (SEDECAL). Data were acquired at 27 µm isotropic voxel size with 720 projections by 360° scan, integration time of 2000 ms with three frames, photon energy of 80 KeV, and current of 450 µA. The duration of imaging time was 10 min per scan and followed by projection correction and volume reconstruction of 3D representation. 3D rendered images of the bones were generated through original volumetric reconstructed images by MicroView software (GE Healthcare). The analysis of the results obtained in the microcomputed tomography was quantified from micro‐computed tomography scans using GE MicroView software v2.2.
Ge microview software v2
Manufactured by GE Healthcare
GE MicroView software v2.2 is a comprehensive imaging and analysis software platform designed to support a wide range of micro-imaging modalities. The software provides essential tools for visualization, quantification, and data management of micro-scale images, enabling users to extract insights from their samples.
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3 protocols using ge microview software v2
1
3D Microcomputed Tomography of Bone
2
Micro-CT analysis of CIA mouse paws
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At day 50 after treatment, mice were sacrificed and hind paws were removed and fixed in 10% neutral buffered formalin. Before micro-CT scanning, paws were washed with running water for 15 min. The imaging of three-dimensional micro-CT was performed with GE eXplore Locus micro-CT scanner (GE Healthcare, Spain). Data were acquired at 27 µm isotropic voxel size with 720 projections by 360° scan, integration time of 2,000 ms with three frames, photon energy of 80 KeV, and current of 450 µA. The duration of imaging time was 100 min per scan and followed by 1 h of projection correction and volume reconstruction of three-dimensional representation. Three-dimensional render images of hind paws were generated through original volumetric reconstructed images by MicroView software (GE Healthcare, Spain). Comparable regions of interest of four metatarsal joints per hind paw (right and left, total eight joints per mouse) were determined. Bone mineral density (BMD = BM/BV mg/cm3) and trabecular mineral density (TMD) were quantified from micro-CT scans using GE MicroView software v2.2. Data are expressed as the BMD and TMD variation in untreated and eASC-treated CIA mice compared to healthy animals.
3
Microstructural Analysis of Rat Femur
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The bone composition of the right femur was determined postmortem by three-dimensional microcomputed tomography (μCT or microCT) analysis. For this purpose, the samples were immersed in physiological serum and frozen at -20 °C. Afterwards, femur samples were fixed in 10% neutral buffered formalin. Comparable regions of interest (ROIs) consisting of the superior epiphysis, the diaphysis and the inferior epiphysis from each rat were selected for analysis. Imaging by threedimensional microcomputed tomography was performed with a CompaCT scanner (SEDECAL, Madrid, Spain). Data were acquired with 720 projections per 360-degree scan, an integration time of 100 ms with three frames, a photon energy of 50 keV, and a current of 100 μA. The duration of the imaging time was 20 min per scan. Three-dimensional rendered images of femur samples were generated through original volumetric reconstructed images by MicroView software (GE Healthcare). The tissue mineral content (TMC), bone mineral content (BMC), tissue mineral density (TMD) and BMD values were quantified from MicroCT scans using GE MicroView software v2.2. These bone composition results were calculated as the sum of the three ROIs selected for each right femur in nine rats per group.
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