Acrilic Acid (AA), ammonium persulfate (APS), bovine serum albumin (BSA), 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC), glutaraldehyde (GA), 3-aminopropyltrimethyloxysilane (APTMS), horseradish peroxidase (HRP), sodium hydroxide (NaOH), sodium cyanoborohydride, N-hydroxysuccinimide (NHS), phosphate buffered saline (PBS), 3,3′,5,5′-tetramethylbenzidine (TMB), N,N′-methylene-bis-acrylamide (BIS), N-isopropylacrylamide (NIPAm), N-tert-butylacrylamide (TBAm), tetramethyl ethylene diamine (TEMED), octopamine hydrochloride, Tween 20, 2-[morpholino] ethanesulfonic acid (MES), and [3-(2-aminoethyl- amino)propyl] trimethoxysilane were purchased from Sigma-Aldrich (Concepción, Chile).
Double-distilled ultra-pure water (Milli-Q, Concepción, Chile) was used in all the experiments. Acetone, ethanol, methanol and toluene were purchased by MERCK. All chemicals and solvents were of analytical or HPLC grade and were used without further purification. Microplates used were Nunclon 96 microwell plates (Thermo Scientific, Concepción, Chile), Amicon centrifugal filter unit (MWCO 30 kDa, Chile).
Double-distilled ultra-pure water (Milli-Q, Concepción, Chile) was used in all the experiments. Acetone, ethanol, methanol and toluene were purchased by MERCK. All chemicals and solvents were of analytical or HPLC grade and were used without further purification. Microplates used were Nunclon 96 microwell plates (Thermo Scientific, Concepción, Chile), Amicon centrifugal filter unit (MWCO 30 kDa, Chile).