Pargyline

Manufactured by Thermo Fisher Scientific

Pargyline is a chemical compound used as a laboratory reagent. It functions as a monoamine oxidase (MAO) inhibitor, which can be utilized in various scientific research applications.

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Lab products found in correlation

Amplex red monoamine oxidase assay kit, by Thermo Fisher Scientific (1 mentions)

2 protocols using pargyline

Measuring MAO-A Activity in Cells and Tissues

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MAO-A enzyme activity was analyzed by Amplex Red Monoamine Oxidase Assay Kit (Invitrogen, #A12214). The lysates from cultured cell and mouse brain tissue (20 μg) were incubated with a working solution of 100 μl containing 400 μM Amplex® Red reagent, 2 U/ml HRP, and 2 mM p-tyramine substrate with MAO-B inhibitor, pargyline (Molecular Probes). The enzyme activity of MAO-A was measured by a fluorescence plate reader in the range of 530–560 nm of excitation and 590 ±10 nm of emission at 37°C for 2 hours in kinetic mode.

Kang S.S., Meng L., Zhang X., Wu Z., Mancieri A., Xie B., Liu X., Weinshenker D., Peng J., Zhang Z, & Ye K. (2022). Tau modification by the norepinephrine metabolite DOPEGAL stimulates its pathology and propagation. Nature structural & molecular biology, 29(4), 292-305.

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Quantification of MAO-A Activity

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Cultured cell or mouse brain tissue lysates (20 μg) were incubated with a working solution of 100 μl containing 400 μM Amplex® Red reagent, 2 U/ml HRP, and 2 mM p-tyramine substrate with MAO-B inhibitor, pargyline (Molecular Probes). The fluorescence of MAO-A activity was measured in a fluorescence plate reader using excitation in the range of 530-560 nm and emission at 590 ±10 nm at 37°C for 2 h in kinetic mode.

Kang S.S., Ahn E.H., Liu X., Bryson M., Miller G.W., Weinshenker D, & Ye K. (2021). ApoE4 Inhibition of VMAT2 in the Locus Coeruleus Exacerbates Tau Pathology in Alzheimer’s Disease. Acta neuropathologica, 142(1), 139-158.

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