Taqman gene expression fam mgb probe system

Differentiation to SYN was defined based on expression of developmental stage-specific markers (Supplemental Table 1). Cells were collected from each developmental stage and total RNA was isolated using the RNeasy plus kit (Qiagen), and reverse transcribed with the high-capacity cDNA reverse transcription kit (Applied Biosystems). Then, cDNA was amplified by performing qPCR with TaqMan^® gene expression. The threshold cycle (Ct) value of 18S rRNA was used as an internal control using the TaqMan^® gene expression FAM/MGB probe system (4333760F, Thermofisher). The Livak method was used to calculate ΔΔCt values and fold change was calculated as 2^−ΔΔCt, as previously described and published.^{59 (link)}