Its microgreens (RM) grown in an open field and harvested at the fully expended green cotyledons stage which was 14 days from seed soaking, washing, and hulling (Abdallah 2008 (link)). Harvested RM was air-dried for 3 days according to previous study (Dzowela et al. 1995 ) and ground into powder. The phytochemical compounds present in RM powder were determined according to a previous method using gas chromatography-mass spectrometry GC/MS technique (Santana et al. 2013 (link)). The analysis was conducted using a GC (Agilent Technology 7890A) coupled with a mass selective detector (MSD, Agilent 7000 Triple Quad) equipped with Agilent HP-5 ms capillary column. The identification of components was based on a comparison of their mass spectra with the authentic compounds and by computer matching with the NIST library as well as by comparison of the fragmentation pattern of the mass spectral data with those registered in the literature. All local, national, or international guidelines and legislation were adhered to use of plants in this study.
Technology 7890a
Manufactured by Agilent Technologies
Sourced in United States
The Agilent 7890A is a gas chromatograph system designed for the analysis of a wide range of chemical compounds. It features an advanced oven, temperature control system, and a variety of detection options to meet the diverse needs of analytical laboratories.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using technology 7890a
1
Phytochemical Profiling of Microgreens
2
Fatty Acid Profiling by GC-MS
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Fatty acid (FA) profiles were analyzed using gas chromatography combined to mass spectrometry (GC-MS) to obtain the proportion of each FA (deuterated or protonated) in ng.mg -1 , and were expressed as relative concentration (weight % relative to total FA contents) as described by Tardy-Laporte et al. [48] . Briefly, starting from three pools of 30 to 60 mg of dry-freezed bacteria, total lipids were extracted using dichloromethane/methanol (2:1 CH 2 Cl 2 /MeOH v/v) and 0.88% KCl solution in a Potter glass homogenizer. Neutral and polar lipids were separated by elution through a silica gel column (30×5 mm) hydrated with 6% water. Polar lipids were transesterified using 2 mL of H 2 SO 4 (2% in MeOH) and 0.8 mL of toluene. Final extracts were diluted in hexane solution and adjusted to a volume of 0.5 mL before GC-MS analysis (Agilent technology-7890A, Santa Clara, CA, USA). FA analyses were performed in parallel on a FAME mix which was used as a standard.
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