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Leitz laborlux s

Manufactured by Leica
Sourced in United States

The Leitz Laborlux S is a microscope designed for use in laboratory settings. It features a sturdy, ergonomic construction and provides high-quality optical performance for a variety of applications.

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3 protocols using leitz laborlux s

1

Nissl Staining of Substantia Nigra

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For the Nissl-staining brain, slices were fixed in 4% formaldehyde in phosphate buffer at pH 7.4. Samples were then washed twice with phosphate buffer, and submerged in 0.5% cresyl violet acid solution for 30 min. Afterwards, samples were washed in distilled water and dehydrated in graded ethanol solutions (70%, 95% and 100%). The slices were finally cleared in xylene (twice, 5 min each). Then, the slices were coverslipped with dibutylphthalate polystyrene xylene (DPX) organic mounting medium (Sigma-Aldrich, Spain). Once dried, histological images at the level of substantia nigra were obtained with a digital camera (DFC425 camera, Leica) coupled to a light microscope (Leitz Laborlux S, Leica).
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2

Quantitative Nerve Fiber Analysis

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Nerves from Lewis rats, including the regenerating tissue from the proximal nerve end or ANA, were harvested and marked with a proximal suture, then stored in 3% glutaraldehyde (Electron Microscopy Sciences; Hatfield, PA) in 0.1 M pH 7.2 phosphate buffer (Fisher Scientific; Fair Lawn, NJ) at 4° C until processing. The explanted tissues were processed and analyzed as described previously32 (link). Briefly, the tissues were post-fixed in 1% osmium tetroxide (Polysciences, Inc., Warrington, PA), and serially dehydrated in 90% ethanol (Thermo Fischer Scientific, Winmill Hill, UK). The tissues were then embedded in grade 502 epoxy (Polysciences, Inc., Warrington, PA) and sectioned to 1 μm with an ultramicrotome. The slides were stained with 1% toluidine blue and analyzed under light microscopy at 1000x (Leitz Laborlux S, Leica; Buffalo Grove, IL) using the IA32 Image Analysis System (Leco; St. Joseph, MI) in order to quantify nerve fiber counts, fiber width, fiber density, and percent neural tissue. One hundred twenty (120) days after the initial nerve injury, the number of myelinated axons within the proximal, middle and distal segment of regenerated tissue, as well as proximal to the initial injury were examined. All analysis was conducted by a blinded observer to the experimental groups.
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3

Trichogramma Species Identification

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Trichogramma species were identified and described after the external and internal morphology analysis of the male. Photographs were taken under a stereo zoom binocular microscope (ARK-zoom Star-VI, AmScope®; Irvine, California, USA) using a Leitz Labor Lux S (Leica®, Wetzlar, Germany) camera. Body measurements were made with oculometer of different magnification powers (10×, 20×, 40×) in the Leitz Labor Lux S.
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