Milliplex map kit human cytokine chemokine growth factor panel a magnetic bead panel

Manufactured by Merck Group

Sourced in Germany

The Milliplex® Map kit Human Cytokine/Chemokine/Growth Factor Panel A Magnetic Bead Panel is a multiplex assay designed to quantify multiple human proteins simultaneously in a single sample. The panel uses magnetic beads coated with analyte-specific antibodies to capture and detect the target proteins.

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Milliplex Map Kit (358 citations) MILLIPLEX MAP (123 citations) Milliplex kit (114 citations) Magnetic beads (109 citations) MILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel (75 citations) MILLIPLEX MAP Human Cytokine/Chemokine Panel (38 citations) Human Cytokine/Chemokine Magnetic Bead Panel (26 citations) MILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel Kit (25 citations) MILLIPLEX MAP Human Cytokine/Chemokine Kit (25 citations) Milliplex Human Cytokine/Chemokine Kit (16 citations)

2 protocols using milliplex map kit human cytokine chemokine growth factor panel a magnetic bead panel

Cytokine Profile Measurement Protocol

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IFN-γ, IL-1β, IL-4, IL-6, IL-10, IL-17A, IP-10 and TNF-α cytokine’s concentration was measured using Milliplex^® Map kit Human Cytokine/Chemokine/Growth Factor Panel A Magnetic Bead Panel (Merck Kgaa, Darmstadt, Germany) and Luminex apparatus following manufacturer instruction (Luminex 200 MAGPIX).
IL-8 was measured by IL-8 Human ELISA (Thermo Fisher Scientific, Waltham, MA, USA) following the manufacturer’s instructions.

Nicolò S., Tanturli M., Mattiuz G., Antonelli A., Baccani I., Bonaiuto C., Baldi S., Nannini G., Menicatti M., Bartolucci G., Rossolini G.M., Amedei A, & Torcia M.G. (2021). Vaginal Lactobacilli and Vaginal Dysbiosis-Associated Bacteria Differently Affect Cervical Epithelial and Immune Homeostasis and Anti-Viral Defenses. International Journal of Molecular Sciences, 22(12), 6487.

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Cytokine Detection in Semen Samples

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Detection and quantification of cytokines in semen were performed using the Milliplex Map kit Human Cytokine/Chemokine/Growth Factor Panel A Magnetic Bead Panel (Merck Kgaa, Darmstadt, Germany)
following the manufacturer's protocols. The plate was analyzed with the Luminex 200 MAGPIX. The limit of sensitivity (MinDc = minimum detectable concentration) and the linear range of detection for the analyzed cytokines are reported in Table S1. Data were analyzed using a 5-parameter logistic curve-fitting method for calculating analyte concentrations in samples.
Semen collection was done after a period of abstinence 2-7 days prior to the collection was requested to all the enrolled patients, according to World Health Organization recommendations. Semen analysis was performed as reported. 9 Quantification of semen leukocytes was done by counting the number of round cells/ml with an improved Neubauer hemocytometer and evaluating the percentage of leukocytes and immature germ cells after May-Grunwald staining of the examined specimen. All analyses were performed by the same team of our academic center.

Morselli S., Sebastianelli A., Liaci A., Zaccaro C., Pecoraro A., Nicoletti R., Manera A., Bisegna C., Campi R., Pollini S., Antonelli A., Lagi F., Coppi M., Baldi E., Marchiani S., Nicolò S., Torcia M., Annunziato F., Maggi M., Vignozzi L., Bartoloni A., Rossolini G.M., Serni S, & Gacci M. (2022). Male reproductive system inflammation after healing from coronavirus disease 2019. Andrology, 10(6).

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