Lipid A extracted from whole cells using the microextraction method was subjected to MALDI-TOF MS analysis (25 (link)). In brief, 2,5-dihydroxybenzoic acid (DHB) matrix solution (10 mg/mL in 80% acetonitrile with 0.1% trifluoroacetic acid) was used for MS. Lipid A extract (2 μL) was mixed with matrix solution (2 μL) deposited on a plate for the vacuum-dried droplet method. The structural spectrum of lipid A was analyzed using the autoflex maX mass spectrometer (Bruker Daltonics, USA) in the negative-ion reflectron mode. The laser repetition rate was 2,000 Hz, and the resulting spectrum was accumulated with an average of 500 shots. The total analysis range of MALDI-TOF MS was m/z 400 to m/z 3,200, and lipid A mass analysis was performed at m/z 1,300 to m/z 2,300.
Autoflex max mass spectrometer
Manufactured by Bruker
Sourced in United States
The Autoflex maX mass spectrometer is a compact, high-performance instrument designed for matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry analysis. It provides accurate mass measurements for a variety of applications, including protein identification and characterization, peptide mapping, and small molecule analysis.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using autoflex max mass spectrometer
1
MALDI-TOF MS Analysis of Lipid A
2
MALDI-TOF MS Analysis of Lipid A
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Lipid A extracted from whole cells using the microextraction method was subjected to MALDI-TOF MS analysis (25 (link)). In brief, 2,5-dihydroxybenzoic acid (DHB) matrix solution (10 mg/mL in 80% acetonitrile with 0.1% trifluoroacetic acid) was used for MS. Lipid A extract (2 μL) was mixed with matrix solution (2 μL) deposited on a plate for the vacuum-dried droplet method. The structural spectrum of lipid A was analyzed using the autoflex maX mass spectrometer (Bruker Daltonics, USA) in the negative-ion reflectron mode. The laser repetition rate was 2,000 Hz, and the resulting spectrum was accumulated with an average of 500 shots. The total analysis range of MALDI-TOF MS was m/z 400 to m/z 3,200, and lipid A mass analysis was performed at m/z 1,300 to m/z 2,300.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!