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Bsa a3912

Manufactured by Merck Group
Sourced in United States

BSA (A3912) is a laboratory-grade bovine serum albumin product supplied by Merck Group. It is a commonly used reagent in various biochemical and cell culture applications.

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2 protocols using bsa a3912

1

Extracellular Matrix Protein Characterization

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Laminin (L2020), cellular fibronectin (F2518), collagen type I (C3867), collagen type IV (C0543), elastin (E6902), e-cadherin (5085), condhroitin (C9819), heparan sulfate (H7640), heparin (SRE0027), condhroitin-4 (27042), plasminogen (P7999), plasma fibronectin (F2006), vitronectin (V8379), fibrinogen (F4883), thrombin (T6884), fetuin (F3004), and BSA (A3912) were obtained from Sigma-Aldrich. Factor H (341274) purified from human serum was purchased from EMD Chemicals. C3b (A114), C4b (A108), C4BP (A109), C5b6 (A122), C6 (A123), C7 (A124), C8 (A125), and C9 (A126) isolated from normal human serum were acquired from Complement Technology.
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2

Albumin and Cafestol Characterization

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HSA (A1653, 96-99%), HSA essentially fatty acid free (A3782, 99%), BSA (A3912, ≥ 96%) were purchased from Sigma-Aldrich Co. (St Louis, MO, USA) and used without further purification.
Their molecular weight were assumed to be 66.478 Da, 66.478 Da and 66.463 Da respectively.
Stock solutions of albumins were prepared by dissolving it in PBS (pH 7.4). All stock solutions were kept at 4 °C and then diluted to the required experimental sample concentrations (1.0 x 10 -6 M). Cafestol and 16OMC were provided by Illycaffè S.p.A. (AromaLab, TS, Italy). Cafestol and 16OMC stock solutions (1.25 mM, 2.5 mM e 5 mM) were prepared in DMSO.
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