Yeast cells were disintegrated with the use of a bead mill (Minilys homogenizer, Bertin Technologies) with the power of 250 V AC / 50-60 Hz, and speed 5 000 rpm. The total working volume of the mill tube was ca 2 ml. Zirconium-glass beads (Bertin Technologies) with diameter of 0.5 cm were used in the experiments. For a single homogenization, 50 mg of the Y. lipolytica cells were used. The cells were resuspended in 0.5 ml Tris-HCl buffer, pH 7.5. 3D beat-beating was carried out in cycles: 10 x 1 min. of homogenization and 0.5 min. in the ice.
Zirconium glass beads
Manufactured by Bertin Technologies
Zirconium-glass beads are a type of laboratory equipment used for various applications. They are made of a combination of zirconium and glass materials. The core function of these beads is to provide a durable and inert medium for applications that require physical agitation or mixing.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using zirconium glass beads
1
Yeast Cell Disruption via Bead Mill
2
Yeast Cell Disintegration Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Yeast cells were disintegrated use of a bead (Minilys homogenizer, Bertin Technologies) with the power of 250 V AC / 50-60 Hz, and speed 5 000 rpm. The total working volume of the mill tube was ca 2 ml. Zirconium-glass beads (Bertin Technologies) with diameter of 0.5 cm were used in the experiments. For a single homogenization, 50 mg of the Y. lipolytica cells were used. The cells were resuspended in 0.5 ml Tris-HCl buffer, pH 7.5. 3D beat-beating was carried out in cycles: 10 x 1 min. of homogenization and 0.5 min. in the ice.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!