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Stattic

Manufactured by Axon Medchem
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Stattic is a chemical compound used as a laboratory research tool. It functions as a specific inhibitor of the STAT3 transcription factor.

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Lab products found in correlation

Smartpool sirna, by Thermo Fisher Scientific (1 mentions) Sb431542, by Bio-Techne (1 mentions) Tofacitinib, by Selleck Chemicals (1 mentions) Belinostat, by Selleck Chemicals (1 mentions) Romidepsin, by MedChemExpress (1 mentions) Trichostatin a, by Selleck Chemicals (1 mentions) Ruxolitinib, by Axon Medchem (1 mentions) Recombinant murine leptin, by Thermo Fisher Scientific (1 mentions) Wortmannin, by Bio-Techne (1 mentions) Shp099, by Cayman Chemical (1 mentions) Ly294002, by Merck Group (1 mentions)

4 protocols using stattic

1

Inhibitors Screen for SARS-CoV-2 PLpro

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For inhibitor treatment, vector control and PLpro-expressing cells were incubated with SB-431542 (TGF-β1 receptor inhibitor) (Tocris Bioscience), YCG063 (ROS inhibitor), SB203580 (p38 MAPK inhibitor), PD98059 (ERK1/2 inhibitor) (Calbiochem), and Stattic (STAT3 inhibitor) (Axon Medchem) at indicated concentrations, and then harvested for Western blotting and real-time RT-PCR, as described above. For the silence of Egr-1 and Rac1, vector control and PLpro-expressing cells were transfected with non-targeting control or Egr-1 siRNA (SMARTpool siRNA) (Thermo Scientific), and then harvested 24 h post transfection for Western blotting and real-time RT-PCR.
Li S.W., Wang C.Y., Jou Y.J., Yang T.C., Huang S.H., Wan L., Lin Y.J, & Lin C.W. (2016). SARS coronavirus papain-like protease induces Egr-1-dependent up-regulation of TGF-β1 via ROS/p38 MAPK/STAT3 pathway. Scientific Reports, 6, 25754.
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2

Modulation of IL-4/IL-13 Receptor Expression

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Unimmunised BALB/c lung suspensions were treated with either 100 nM of small molecule STAT6 inhibitor (Axon Medchem) or 20 μM Stattic (small molecule STAT3 inhibitor) in PBS overnight followed by low (100 pg/ml) or high (10,000 pg/ml) IL-13 stimulation for 3 h or 0.5 h before evaluation of IL-4 and IL-13 receptor expression on lung DCs using flow cytometry as described above. Biologically relevant inhibitor concentrations were used in this study as reported previously28 (link),60 (link).
Roy S., Liu H.Y., Jaeson M.I., Deimel L.P, & Ranasinghe C. (2020). Unique IL-13Rα2/STAT3 mediated IL-13 regulation detected in lung conventional dendritic cells, 24 h post viral vector vaccination. Scientific Reports, 10, 1017.
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3

Inhibitor Screening for Epigenetic Targets

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Chidamide was provided by Shenzhen Chipscreen Biosciences Ltd. SAHA was obtained from Sigma–Aldrich. Trichostatin A (TSA), belinostat and tofacitinib were purchased from Selleckchem. Romidepsin was obtained from MedChemExpress. Ruxolitinib and Stattic were purchased from Axon Medchem and Millipore, respectively.
Chen J., Zuo Z., Gao Y., Yao X., Guan P., Wang Y., Li Z., Liu Z., Hong J.H., Deng P., Chan J.Y., Cheah D.M., Lim J., Chai K.X., Chia B.K., Pang J.W., Koh J., Huang D., He H., Sun Y., Liu L., Liu S., Huang Y., Wang X., You H., Saraf S.A., Grigoropoulos N.F., Li X., Bei J., Kang T., Lim S.T., Teh B.T., Huang H., Ong C.K, & Tan J. (2023). Aberrant JAK-STAT signaling-mediated chromatin remodeling impairs the sensitivity of NK/T-cell lymphoma to chidamide. Clinical Epigenetics, 15, 19.
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4

Tyrode Solution for Taste Cell Assay

Cited 2 times
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Tyrode solution contained (in mM): NaCl, 140; KCl, 5; CaCl2, 1; MgCl2, 1; NaHCO3, 5; HEPES, 10; Glucose, 10; sodium pyruvate, 10; pH adjusted to 7.4 with NaOH. Sucrose (500mM) was used as a sweet tastant. Recombinant murine leptin (20 ng/ml; PeproTech, Cat# 450-31), wortmannin (10 nM; Tocris Bioscience, Cat# 1,232), LY294002 (30 μM; Sigma-Aldrich, Cat# L9908), SHP099 (10 μM; Cayman Chemical, Cat# 20,000), and stattic (10 μM; Axon Medchem, Cat# 2,314) were applied from the basolateral side of TCs. Other chemicals were purchased from Wako Pure Chemical Industries or Nakarai Tesque. These concentrations of agents were selected as used in previous studies (Chen et al., 2016 (link); Mirshamsi et al., 2004 (link); Schust et al., 2006 (link); Yoshida et al., 2015 (link)).
Yoshida R., Margolskee R.F, & Ninomiya Y. (2021). Phosphatidylinositol-3 kinase mediates the sweet suppressive effect of leptin in mouse taste cells. Journal of neurochemistry, 158(2), 233-245.
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