The largest database of trusted experimental protocols

4 protocols using stattic

1

Inhibitors Screen for SARS-CoV-2 PLpro

Check if the same lab product or an alternative is used in the 5 most similar protocols
For inhibitor treatment, vector control and PLpro-expressing cells were incubated with SB-431542 (TGF-β1 receptor inhibitor) (Tocris Bioscience), YCG063 (ROS inhibitor), SB203580 (p38 MAPK inhibitor), PD98059 (ERK1/2 inhibitor) (Calbiochem), and Stattic (STAT3 inhibitor) (Axon Medchem) at indicated concentrations, and then harvested for Western blotting and real-time RT-PCR, as described above. For the silence of Egr-1 and Rac1, vector control and PLpro-expressing cells were transfected with non-targeting control or Egr-1 siRNA (SMARTpool siRNA) (Thermo Scientific), and then harvested 24 h post transfection for Western blotting and real-time RT-PCR.
+ Open protocol
+ Expand
2

Modulation of IL-4/IL-13 Receptor Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Unimmunised BALB/c lung suspensions were treated with either 100 nM of small molecule STAT6 inhibitor (Axon Medchem) or 20 μM Stattic (small molecule STAT3 inhibitor) in PBS overnight followed by low (100 pg/ml) or high (10,000 pg/ml) IL-13 stimulation for 3 h or 0.5 h before evaluation of IL-4 and IL-13 receptor expression on lung DCs using flow cytometry as described above. Biologically relevant inhibitor concentrations were used in this study as reported previously28 (link),60 (link).
+ Open protocol
+ Expand
3

Inhibitor Screening for Epigenetic Targets

Check if the same lab product or an alternative is used in the 5 most similar protocols
Chidamide was provided by Shenzhen Chipscreen Biosciences Ltd. SAHA was obtained from Sigma–Aldrich. Trichostatin A (TSA), belinostat and tofacitinib were purchased from Selleckchem. Romidepsin was obtained from MedChemExpress. Ruxolitinib and Stattic were purchased from Axon Medchem and Millipore, respectively.
+ Open protocol
+ Expand
4

Tyrode Solution for Taste Cell Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tyrode solution contained (in mM): NaCl, 140; KCl, 5; CaCl2, 1; MgCl2, 1; NaHCO3, 5; HEPES, 10; Glucose, 10; sodium pyruvate, 10; pH adjusted to 7.4 with NaOH. Sucrose (500mM) was used as a sweet tastant. Recombinant murine leptin (20 ng/ml; PeproTech, Cat# 450-31), wortmannin (10 nM; Tocris Bioscience, Cat# 1,232), LY294002 (30 μM; Sigma-Aldrich, Cat# L9908), SHP099 (10 μM; Cayman Chemical, Cat# 20,000), and stattic (10 μM; Axon Medchem, Cat# 2,314) were applied from the basolateral side of TCs. Other chemicals were purchased from Wako Pure Chemical Industries or Nakarai Tesque. These concentrations of agents were selected as used in previous studies (Chen et al., 2016 (link); Mirshamsi et al., 2004 (link); Schust et al., 2006 (link); Yoshida et al., 2015 (link)).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!