Data were recorded with an Axoclamp-2B amplifier (Axon Instruments Inc., Union City, CA, USA). Extracellular data were filtered at 0.001–0.4 kHz and intracellular signals were low pass filtered at 2 kHz using Neurolog filters (Digitimer, Welwyn Garden City, Herts, UK). Mains noise was subtracted from the signal with a Humbug (Digitimer). Data were re-digitised at 10 kHz using an ITC-16 interface (Digitimer). Data were recorded using Axograph 4.6 software (Axon Instruments Inc.) and saved for further analysis.
Axograph 4
Axograph 4.6 is a software application designed for data analysis and visualization. It provides a range of tools for the processing and analysis of experimental data, including electrophysiological recordings, waveforms, and time-series data.
5 protocols using axograph 4
Extracellular and Intracellular Recordings from Prefrontal Cortex Neurons
Data were recorded with an Axoclamp-2B amplifier (Axon Instruments Inc., Union City, CA, USA). Extracellular data were filtered at 0.001–0.4 kHz and intracellular signals were low pass filtered at 2 kHz using Neurolog filters (Digitimer, Welwyn Garden City, Herts, UK). Mains noise was subtracted from the signal with a Humbug (Digitimer). Data were re-digitised at 10 kHz using an ITC-16 interface (Digitimer). Data were recorded using Axograph 4.6 software (Axon Instruments Inc.) and saved for further analysis.
Intracellular and Extracellular Recordings of Gamma Oscillations in CA3
Extracellular recording of gamma oscillations
Gamma frequency oscillations were evoked using the cholinergic agonist carbachol (10 µM) and field traces were recorded from the border between stratum radiatum and stratum lacunosum moleculare in CA3. Data were recorded with an Axoclamp-2B amplifier (Axon Instruments Inc., Union City, CA, USA). Extracellular data were filtered at 0.001-0.4 kHz and intracellular signals were low pass filtered at 2 kHz using Bessel filters. Mains noise was subtracted from the signal with a Humbug (Digitimer, Welwyn Garden City, Herts, UK). Data were redigitised at 10 kHz using an ITC-16 interface (Digitimer, Welwyn Garden City, UK).
Data were recorded and analysed using Axograph 4.6 software (Axon Instruments Inc., Union City, CA, USA). Further offline analysis was performed using MATLAB (Mathworks, Natick, USA).
Electrophysiological Analysis of Synaptic Responses
Measuring Deactivation and Desensitization of GluA1
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