The sample processing encompassed the blood analysis in the Hilab System and the conventional hematology analyzer (XE-2100, Sysmex Corporation, Japan) for reference values establishment. For statistical analysis, the study calculated and displayed the Passing–Bablok regression, Student t-test, bias, and the Bland–Altman plot of each CBC analyte. In addition, the study evaluated the Hilab System accuracy compared to Sysmex XE-2100 data across the clinical range. Thus, for each analyte, the data provided by each CBC methodology were transformed into the standard reference interval and compared. Following this, through a confusion matrix, the parameters of specificity, sensitivity, balanced accuracy, and kappa coefficient were evaluated. As this was a double-blind study, different professionals employed the Hilab device and the conventional analyzer analysis. Additionally, all biological samples collected were single-use for this study and discarded after the processing, following the potentially infected samples’ standard procedure.
Xe 2100
Manufactured by Sysmex
Sourced in Japan, Germany, United Kingdom, United States, Brazil
The XE-2100 is a hematology analyzer designed for automated blood cell analysis. It provides comprehensive analysis of various blood cell types, including red blood cells, white blood cells, and platelets. The XE-2100 is capable of performing a wide range of hematological tests and measurements to support clinical decision-making.
Automatically generated - may contain errors
Lab products found in correlation
Xe 5000, by Sysmex (9 mentions)
Cobas 8000, by Roche (8 mentions)
Ca 1500, by Sysmex (5 mentions)
Cobas integra 800, by Roche (5 mentions)
Advia 1800, by Siemens (4 mentions)
Dimension vista 1500, by Siemens (4 mentions)
Modular p800, by Roche (3 mentions)
Cobas 6000, by Roche (3 mentions)
Au5400, by Beckman Coulter (3 mentions)
Xn 9000, by Sysmex (3 mentions)
Cobas 6000 analyzer series, by Roche (3 mentions)
Acs 180, by Bayer (3 mentions)
Acl top, by Mitsubishi (3 mentions)
Microvette cb 300 k2e, by Sarstedt (2 mentions)
Xs 1000i, by Sysmex (2 mentions)
Au2700, by Beckman Coulter (2 mentions)
Gm 1000, by Seca (2 mentions)
Arkray4030, by Sysmex (2 mentions)
Uf 1000i, by Sysmex (2 mentions)
Immunocap, by Thermo Fisher Scientific (2 mentions)
393 protocols using xe 2100
1
Comparative Performance Evaluation of Hilab System
2
Automated Platelet Immaturity Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
We collected 3 mL of peripheral blood from each patient in K2-EDTA anticoagulant bottles (Becton Dickinson, Franklin Lakes, NJ, USA) on the day of thrombocytopenia confirmation. Samples were analyzed using the Sysmex XE-2100 to quantify IPF and routine complete blood count within 8 hours after collection. The Sysmex XE-2100 is a fully-automated hematology analyzer using fluorescent dye and a semiconductor diode laser beam system. Two fluorescent dyes (polymethine and ozazine) penetrate into the cells, staining the RNA in the platelets. The stained cells are passed through a semiconductor diode laser beam and resulting forward scatter light (cell volume) and fluorescence intensity (RNA content) measured. The immature platelet fractions are distinguished by the intensity of their fluorescence because the youngest platelets contain a greater amount of RNA [15 (link)]. The IPF is expressed as a proportional value of the total optical platelet count. Several studies have demonstrated the stability and reproducibility of the IPF.
We also estimated other variables that could affect thrombocytopenia by logistic regression analysis, including hemoglobin, platelet count, white blood cell counts, reticulocyte count, protein, albumin, bilirubin, prothrombin, activated partial thromboplastin time, ferritin, lactate dehydrogenase (LDH), blood urea nitrogen, creatinine, and C-reactive protein.
We also estimated other variables that could affect thrombocytopenia by logistic regression analysis, including hemoglobin, platelet count, white blood cell counts, reticulocyte count, protein, albumin, bilirubin, prothrombin, activated partial thromboplastin time, ferritin, lactate dehydrogenase (LDH), blood urea nitrogen, creatinine, and C-reactive protein.
3
Platelet Characteristics in Healthy Individuals
4
Comprehensive Biomarker Analysis Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Leucocyte levels were conducted on a Sysmex SE9000 with a coefficient of variation (CV) of 5%. Neutrophil, eosinophil, basophil, lymphocyte, and monocyte levels on a Sysmex XE-2100 with a CV of 6%, 12%, 6%, 6%, and 15%, respectively. CRP was measured on a Cobas 8000, c702 modul with a CV of 6%, whereas orosomucoid was measured on a Cobas 8000, c502 modul with a CV of 9%. Ferritin was measured on a Modular E-modul with a CV of 7% and PTH was measured on a Cobas 8000 (Roche) with a CV of 7%. Measurements of hemoglobin (CV2%) and thrombocytes were both conducted on a Sysmex XE-2100. The vitamin D measurements of 25(OH)D3 (CV < 10%) and 1,25(OH)2D3 (< 18%) levels were measured with an isotope-dilution liquid chromatography-tandem mass spectrometry (LC-MS/MS).
5
Analyzing Immature Granulocytes in Sepsis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The study parameters were collected during the first routine blood analysis sampling at the emergency department of patients with a suspicion of infection. Blood samples were obtained by venipuncture in EDTA vacutainer tubes. Blood cultures were collected by two separate vein punctures for all patients with suspicion of sepsis.
The IG percentage was calculated with an automated hematology analyzer Sysmex XE 2100. The IG measurement that includes promyelocytes, myelocytes, and metamyelocytes was performed in the differential channel of the Sysmex XE 2100. A specific lysing reagent causes mature WBC membranes disruption, leaving bare nuclei, but immature myeloid cells remain intact due to low cell membrane lipid content. The increased permeability of leucocytes allows for a polymethine dye to enter the cells with high affinity for nuclei acid. The cells are analysed by nucleic acid fluorescence and side scatter.
The IG percentage is defined as the percentage of the total WBC count [10 (link),12 (link)]. CRP levels were measured by the latex method (Cobas Integra; Roche Diagnostics, Risch-Rotkreuz, Switzerland), the lowest assay sensitivity being 0.085 mg/L.
The IG percentage was calculated with an automated hematology analyzer Sysmex XE 2100. The IG measurement that includes promyelocytes, myelocytes, and metamyelocytes was performed in the differential channel of the Sysmex XE 2100. A specific lysing reagent causes mature WBC membranes disruption, leaving bare nuclei, but immature myeloid cells remain intact due to low cell membrane lipid content. The increased permeability of leucocytes allows for a polymethine dye to enter the cells with high affinity for nuclei acid. The cells are analysed by nucleic acid fluorescence and side scatter.
The IG percentage is defined as the percentage of the total WBC count [10 (link),12 (link)]. CRP levels were measured by the latex method (Cobas Integra; Roche Diagnostics, Risch-Rotkreuz, Switzerland), the lowest assay sensitivity being 0.085 mg/L.
6
Evaluating Blood Markers after Esophageal ESD
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Patients with EEC after ESD were required to abstain from food and water for 3 days. Patients should be given acid suppression and fluid rehydration therapy within 3 days. After 3 days, the patients were discharged, with no obvious discomfort after taking liquid food. All participants underwent a complete blood count (CBC) and an automatic differential count. Peripheral fasting blood samples (2 mL each) were collected from the cubital vein on the morning of the third day after ESD. The CBC tests were conducted within 4 hours. The counts of peripheral blood white blood cells, neutrophils, lymphocytes, monocytes, and platelets were detected using a blood counting instrument (Sysmex XE-2100, Sysmex Corporation, Japan). Then, different ratios, including the NLR, PLR, and MLR, were determined. During the test, all procedures were conducted in strict accordance with the operating procedures, and all experimental reagents (Sigma, Japan) were matched with the instrument (Sysmex XE-2100, Sysmex Corporation, Japan).
7
Venous Blood Analysis for Surgical Patients
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Venous blood samples after an 10-hour overnight fasting were collected from the individuals within 1 week prior to surgery. White blood cell (WBC), haemoglobin, and platelet indices were measured by an autoanalyzer (Sysmex XE-2100, Kobe, Japan). The whole blood samples were collected in EDTA-containing tubes, and all samples were processed within 30 minutes after blood collection.
8
Immune Markers in Blood Cell Counts
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Neutrophil, lymphocyte, platelet, and monocyte counts were performed automatically using a Sysmex XE-2100 or XE-5000 automated hematology system (Sysmex Co., Kobe, Japan). ALC, NLR, PLR, and LMR were calculated from blood cell counts prior to administering PB therapy, and the cut-off values for these markers were set in accordance with previous studies as follows: 1500/μL for ALC, 3 for NLR, 300 for PLR, and 3 for LMR12 (link),13 (link),15 (link),20 (link). All patients were divided into “low” and “high” groups according to the cut-off values, respectively: low ALC (≤ 1500/μL, n = 87), high ALC (> 1500/μL, n = 27); low NLR (≤ 3, n = 60), high NLR (> 3, n = 54); low PLR (≤ 300, n = 82), high PLR (> 300, n = 32); and low LMR (≤ 3, n = 56), high LMR (> 3, n = 58). Neutrophil, lymphocyte, platelet, and monocyte counts are routinely measured in clinical practice during treatment and the systemic immunity markers are easily calculated from these blood cell counts. Therefore, these markers can be measured and analyzed easily and inexpensively without additional equipment, software, or personnel specialized in analyzing the results.
9
Monitoring Iron Metabolism in Athletes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Alterations of iron metabolism (hepcidin, iron, ferritin, transferrin, and sTfR) were analyzed in venous serum samples. Blood samples were drawn after an overnight fast (starting 08:00 pm until 06:00 am) from the antecubital vein in the seated position ≥14 h after the last training session in the morning before breakfast. No rower suffered from anemia (hemoglobin [Hb] <120 g/l), and no athlete was classified as iron-deficient (serum ferritin levels <30 μg/l) (Clénin et al., 2015 (link)). Hemoglobin levels were measured via photometric testing with XE-2100, Sysmex. Ferritin and sTfR were measured by immunoturbidimetric testing (Cobas 6000, Roche). Transferrin was measured through immunoturbidimetric testing (Cobas 8000, Roche), and serum iron was determined by photometric testing (Cobas 6000, Roche). Total hepcidin was analyzed in serum samples using a commercial ELISA (USCN cat.-nr. 979hu). The ferritin index was calculated by dividing serum levels of the sTfR by log ferritin (sTFR/log ferritin). The transferrin saturation was calculated using the following formula: Tf-sat [%] = serum-iron [μg/dl]/serum-transferrin [mg/dl] × 70.9. To estimate changes in plasma volume, hematocrit was measured in capillary samples taken from the hyperaemized earlobe via centrifugation for 10 min at 10,000 rpm (Laborfuge A, Heraeus, Buckinghamshire, United Kingdom).
10
Lipid Profile Analysis in TA Patients
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Early in the morning, venous blood samples were obtained from fasting TA patients and healthy controls. Our team previously compared methods for measuring sdLDL‐C,36 and a Beckman AU5400 (US) automatic biochemical analyzer was used in the present study to measure novel and traditional lipid parameters and biochemical indicators, including the sdLDL‐C, Lp(a), total cholesterol (TC), LDL‐C, HDL‐C, and triglyceride (TG) concentrations within 5–6 h after sample collection. The Sysmex XE‐2100 was used to determine complete blood counts. The experiments were accomplished following the manufacturer's instructions. In addition, all measurements were analyzed using the continuous monitoring method, and appropriate quality control was carried out before these analyses.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!