Model s 2260n

Manufactured by Hitachi

Sourced in Japan

The Hitachi Model S-2260N is a scanning electron microscope (SEM) designed for high-resolution imaging of samples. It features a tungsten filament electron source and a resolution of up to 3.0 nm. The instrument is capable of magnifications ranging from 20x to 300,000x.

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Lab products found in correlation

Image pro plus software, by Media Cybernetics (1 mentions) Hummer 6, by Anatech (1 mentions)

2 protocols using model s 2260n

Scanning Electron Microscopy of Chitosan Fibers and Scaffolds

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The chitosan fibers and scaffolds were observed under scanning electron microscopy. Chitosan fibers were allowed to dry at room temperature and used for imaging. Different types of lyophilized scaffolds were sectioned using a sharp scalpel. Fibers and cross sections of scaffolds were mounted on a stub with double sticky tape and colloidal graphite. Samples were then sputter-coated with gold prior to examination under scanning electron microscopy (SEM; Model S-2260N, Hitachi Co. Ltd., Tokyo, Japan).

Zakhem E, & Bitar K.N. (2015). Development of Chitosan Scaffolds with Enhanced Mechanical Properties for Intestinal Tissue Engineering Applications. Journal of Functional Biomaterials, 6(4), 999-1011.

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Electrospun Scaffold Ultrastructure Analysis

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The ultrastructure of the electrospun scaffolds was observed using a scanning electron microscopy (SEM) (Model S-2260N; Hitachi Co. Ltd, Tokyo, Japan). The dried scaffolds were coated with a thin gold layer using a sputtering system (Hummer 6.2, Anatech Ltd, Denver, NC, USA). The SEM images were acquired at an accelerating voltage of 20–25 kV and a 15 cm working distance. IMAGE-PRO PLUS software (Media Cybernetics, Bethesda, MD, USA) was used to measure the fibre diameters of scaffolds. Three SEM images were taken at different locations for each sample and 20 fibres were randomly selected for measurements. In the current experiments, a 5% PCL/collagen solution was used, resulting in scaffolds with fibres of about 250 nm diameter (see the Supporting Information, Figure S1)

Niu G., Sapoznik E., Lu P., Criswell T., Mohs A.M., Wang G., Lee S.J., Xu Y, & Soker S. (2014). Fluorescent imaging of endothelial cells in bioengineered blood vessels: the impact of crosslinking of the scaffold. Journal of tissue engineering and regenerative medicine, 10(11), 955-966.

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