Follicle dpc growth medium

hDPCs at P5 to P13 were obtained (c-12071; PromoCell, Heidelberg, Germany) and cultured in Follicle DPC Growth Medium (PromoCell) with 0.1% Gibco^™ antibiotic-antimycotic (Thermo Fisher Scientific, Waltham, MA, USA). Cells were maintained in a humidified incubator at 37° C under 5% CO₂.

Dimethyl sulfoxide (DMSO), 100× penicillin-streptomycin, chloroform solution, and eosin Y solution were purchased from Sigma-Aldrich (Saint Louis, MO, USA). A hematoxylin solution and a dihydrotestosterone enzyme-linked immunosorbent assay (ELISA) kit were purchased from Abcam, Inc. (Cambridge, MA, USA). Dulbecco’s phosphate-buffer saline (DPBS) was purchased from Corning, Inc. (Corning, NY, USA). DMEM, 0.25% Trypsin-EDTA, and TRIzol were purchased from Thermo Fisher Scientific, Inc. (Waltham, MA, USA). Follicle DPC growth medium was purchased from PromoCell GmbH (Heidelberg, Germany). EZ-Cytox cell viability assay reagent was purchased from DoGenBio, Inc. (Seoul, Republic of Korea). HelixCript Thermo Reverse Transcriptase and RNase inhibitor were purchased from Nanohelix, Inc. (Daejeon, Republic of Korea). A TB Green^® Premix Ex Taq kit was purchased from Takara Korea Biomedical, Inc. (Seoul, Republic of Korea). Collagen type I-coated wells were purchased from SPL, Inc. (Gyenggi, Republic of Korea). Pansidil was purchased from Dongkook Pharmaceutical, Inc. (Seoul, Republic of Korea).