2.5.2 autosampler

Manufactured by Agilent Technologies

Sourced in United States

The 2.5.2 autosampler is a laboratory equipment product designed to automatically introduce liquid samples into an analytical instrument, such as a chromatograph or spectrometer, for analysis. The core function of the autosampler is to precisely and consistently deliver samples to the instrument, allowing for high-throughput and unattended sample processing.

Automatically generated - may contain errors

Lab products found in correlation

Agilent 7890a gas chromatograph, by Agilent Technologies (4 mentions) Agilent 5975c quadrupole mass spectrometer, by Agilent Technologies (3 mentions) J w vf 5ms column, by Agilent Technologies (1 mentions) Rp amide, by Agilent Technologies (1 mentions) Qqq6490 mass spectrometer, by Agilent Technologies (1 mentions)

4 protocols using 2.5.2 autosampler

GC-MS Analysis of TMS-Derivatized Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

The GC-MS system used comprised of a Gerstel 2.5.2 autosampler, a 7890A Agilent gas chromatograph and a 5975C Agilent quadrupole mass spectrometer (Agilent, Santa Clara, CA, United States). The mass spectrometer was tuned according to the manufacturer’s recommendations using tris-(perfluorobutyl)-amine (CF43).
Gas chromatography was performed on a 30 m Agilent J & W VF-5MS column with 0.25 μm film thickness and 0.25 mm internal diameter with a 10 m Integra guard column. The injection temperature (Inlet) was set at 250°C, the MS transfer line at 280°C, the ion source adjusted to 230°C, and the quadrupole at 150°C. He was used as the carrier gas at a flow rate of 1 mL min^–1.
The analysis of TMS-derivatized samples was performed under the following temperature program; start at injection 70°C, a hold for 1 min, followed by a 7°C min^–1 oven temperature ramp to 325°C and a final 6 min heating at 325°C. Mass spectra were recorded at 2.66 scans.s^–1 with an 50–600 m/z scanning range.

Nguyen V.L., Palmer L., Roessner U, & Stangoulis J. (2019). Genotypic Variation in the Root and Shoot Metabolite Profiles of Wheat (Triticum aestivum L.) Indicate Sustained, Preferential Carbon Allocation as a Potential Mechanism in Phosphorus Efficiency. Frontiers in Plant Science, 10, 995.

+ Open protocol

+ Expand

Barley Metabolite Extraction and GC-MS Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Fifty (50 ± 2) mg of fresh frozen root tissue harvested from both varieties of barley was extracted in 500 μl of 100% MeOH (Scharlau, city, country), containing 4% v/v of [¹³C₆] Sorbitol/[¹³C₅¹⁵N] Valine (0.5 mg mL⁻¹). The samples were prepared according to the instructions in Gupta et al. (2021) (link). Pooled blank quality control (PBQC) samples were prepared by combining 50 μl aliquots from each sample solution into a single sample. A GC–MS system comprising of a Gerstel 2.5.2 autosampler, a 7890A Agilent gas chromatograph, and a 5975C Agilent quadrupole mass spectrometer (Agilent, Santa Clara, United States) with an electron impact (EI) ion source was used to analyse samples with an injection volume of 1 μl for each derivatized sample. Instrumental settings were adapted from Dias et al. (2015) (link). Calibration of polar metabolites was done using serial concentrations of calibration authentic standards. Detailed instrumental parameters are provided in Supporting Experimental Materials and Methods.

Gupta S.V., Smith P.M., Natera S.H, & Roessner U. (2022). Biochemical Changes in Two Barley Genotypes Inoculated With a Beneficial Fungus Trichoderma harzianum Rifai T-22 Grown in Saline Soil. Frontiers in Plant Science, 13, 908853.

+ Open protocol

+ Expand

Lipidomics and Fatty Acid Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Please see the Supporting information (S1 File) for a complete description of lipidomics materials and methods. Lipids for LC-MS analysis were extracted using a modified Bligh Dyer extraction protocol. Lipids were analyzed using Agilent LC 1290 binary pump coupled with Ascentis Express RP amide (50 ×2.1 mm, 1.8u), and separated lipid species were detected using Agilent QQQ 6490 mass spectrometer, using multiple reaction monitoring (MRM) as previously published [6 (link)].
For GC-MS based fatty acid analysis, dried samples and dried fatty acid calibration mix were derivatised with 5 μL of Meth-Prep^™ II (Grace Davison Discovery). The samples were then analyzed on a GC-MS system comprised of a Gerstel 2.5.2 Autosampler, a 7890A Agilent gas chromatograph and a 5975C Agilent quadrupole mass spectrometer (Agilent, Santa Clara, USA) [18 , 19 (link)].

Vasiljevski E.R., Houweling P.J., Rupasinghe T., Kaur T., Summers M.A., Roessner U., Little D.G, & Schindeler A. (2020). Evaluating modified diets and dietary supplement therapies for reducing muscle lipid accumulation and improving muscle function in neurofibromatosis type 1 (NF1). PLoS ONE, 15(8), e0237097.

+ Open protocol

+ Expand

GC-QqQ-MS Analysis of Derivatized Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols

1 µl of derivatised sample was injected into a GC-QqQ-MS system comprised of a Gerstel 2.5.2 Autosampler, a 7890A Agilent gas chromatograph and a 7000 Agilent triple-quadrupole MS (Agilent, Santa Clara, USA) with an electron impact (EI) ion source. The GC was operated in constant flow mode with helium as carrier gas. The MS was adjusted according to the manufacturer's recommendations using tris-(perfluorobutyl)-amine (CF43). A J&W Scientific VF-5MS column (30 m long with 10 m guard column, 0.25 mm inner diameter, 0.25 µm film thickness) was used. The injection temperature was set at 250 °C, the MS transfer line at 290 °C, the ion source was adjusted to 230 °C and the quadrupole at 150 °C. Helium was used as carrier gas at flow rate of 1 ml min -1 . Nitrogen (UHP 5.0) was used as collision cell gas at flow rate of 1.5 ml min -1 . Helium (UHP 5.0) was used as quenching gas at a flow rate of 2.25 ml min -1 . Gain factor for the triple axis detector was set at 2. Derivatised sample was injected into the column at 100 °C followed by 1 min hold, followed by a ramp of 25 °C min -1 to 325 °C.

Casartelli A., Melino V.J., Baumann U., Riboni M., Suchecki R., Jayasinghe N.S., Mendis H., Watanabe M., Erban A., Zuther E., Hoefgen R., Roessner U., Okamoto M, & Heuer S. (2019). Opposite fates of the purine metabolite allantoin under water and nitrogen limitations in bread wheat. Plant molecular biology, 99(4-5).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!