Log In Sign up
The largest database of trusted experimental protocols

Anti jagged1

Manufactured by R&D Systems
Visit Supplier

Anti-JAGGED1 is a recombinant protein that binds to and neutralizes the JAGGED1 ligand. JAGGED1 is a transmembrane protein that plays a critical role in the Notch signaling pathway. The Anti-JAGGED1 protein can be used in research applications to study the Notch signaling pathway and its biological functions.

Automatically generated - may contain errors

Lab products found in correlation

Facscalibur, by BD (2 mentions) Facsaria 2 cytometer, by BD (2 mentions) Anti notch1, by Thermo Fisher Scientific (2 mentions) Versene, by Thermo Fisher Scientific (2 mentions) Anti latency associated peptide, by Thermo Fisher Scientific (2 mentions) Anti p akt, by Cell Signaling Technology (2 mentions) Transwell insert, by Corning (1 mentions) Ns 398, by Cayman Chemical (1 mentions) Rpmi 1640, by Miltenyi Biotec (1 mentions) Il 15, by Miltenyi Biotec (1 mentions) Anti β actin, by Merck Group (1 mentions) Anti ng2, by Merck Group (1 mentions) Anti c myc, by Merck Group (1 mentions) Anti ph3, by Merck Group (1 mentions) Anti akt, by Cell Signaling Technology (1 mentions) Anti ephb4, by R&D Systems (1 mentions) Anti smad4, by Cell Signaling Technology (1 mentions) Anti p pten, by Cell Signaling Technology (1 mentions) Anti foxo1, by Cell Signaling Technology (1 mentions) Anti pten, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

Jagged1 (11 citations) Anti-Jagged1-FITC (2 citations)

6 protocols using anti jagged1

1

Modulation of NK cell function by DSC

Check if the same lab product or an alternative is used in the 5 most similar protocols
NK cells were cultured in RPMI-1640 10% FCS and 10 ng/mL IL15 (Miltenyi Biotec), in 24-well flat bottom plates either in the absence or in the presence of DSC (NK/DSC ratio: 5/1). At the indicated time intervals NK cells were harvested and analyzed. When indicated, 0,25 mM 1-MT (Sigma–Aldrich) and/or 5 uM NS-398 (Cayman Chemicals) and 10 µg/ml anti-Jagged-1 (R&D systems) were added at the onset of co-cultures. In some experiments, transwell inserts (Corning incorporated, 6,5 mm/0,4 µm) were used to separate NK from DSCs.
Croxatto D., Vacca P., Canegallo F., Conte R., Venturini P.L., Moretta L, & Mingari M.C. (2014). Stromal Cells from Human Decidua Exert a Strong Inhibitory Effect on NK Cell Function and Dendritic Cell Differentiation. PLoS ONE, 9(2), e89006.
+ Open protocol
+ Expand
2

Flow Cytometric Analysis of Notch Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were washed once with cold PBS, prior to dissociation with Versene (Life Technologies), washed twice more in PBS / 0.1% Fetal calf serum, blocked in 1% mouse or rabbit serum before incubation with combinations of the following fluorochrome-conjugated antibodies: anti-NOTCH1 (Ebioscience, 17-9889, 1:50); anti-JAGGED1 (R&D systems, FAB1726A, 1:8); anti-latency associated peptide (Ebioscience, 17-9829, 1:20). Cells were then washed twice more, before fixation with 4% PFA and analysis on a FACSCalibur (Becton Dickenson). Flow-based cell sorting was conducted on a FACSAria II cytometer (Becton Dickenson). Flow data was analysed with FlowJo v10.
Hoare M., Ito Y., Kang T.W., Weekes M.P., Matheson N.J., Patten D.A., Shetty S., Parry A.J., Menon S., Salama R., Antrobus R., Tomimatsu K., Howat W., Lehner P.J., Zender L, & Narita M. (2016). NOTCH1 mediates a switch between two distinct secretomes during senescence. Nature cell biology, 18(9), 979-992.
+ Open protocol
+ Expand
3

Immunodetection and Western Blotting Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols
For immunodetection: anti-Smad4 (#38454, 1:200, Cell Signaling), anti-Jagged1 (AF599, 1 μg/ ml, R&D systems), anti-α-SMA (CY3-SMA, #C6198, 1:200, Sigma), anti-pS6 (#5364, 1:200, Cell Signaling), IB4 (#121412, 10 μg/ml, Life Technologies), anti-FOXO1 (#2880, 1:100, Cell Signaling), anti-cMYC (#6340, 1:100, Millipore), anti-KLF4 (#AF3158, 1:200, R&D systems), anti-NG2 (#Ab5320; 1:200, EMD Millipore), anti-PH3 (#06570; 1:500, EMD Millipore), anti-EPHB4 (#AF446, 1:100, R&D systems), anti-UNC5B (#AF1006, 1:100, R&D systems) anti-NRP2 (#AF567, 1:100, R&D systems).
For western blotting: anti-ALK1 (7R-49334, 1:1000, Fitzgerald), anti-Smad4 (#38454, 1:1.000, Cell Signaling), anti-pAKT (#4060, 1:1.000, Cell Signaling), anti-AKT (#9272, 1:1.000, Cell Signaling), anti-pPTEN (#9549, 1:1.000, Cell Signaling) and anti-PTEN (#9188, 1:1.000, Cell Signaling) and anti β-ACTIN (#A1978 1:3000, Sigma).
Appropriate secondary antibodies were fluorescently labelled (Alexa Fluor donkey anti-rabbit, Alexa Fluor donkey anti-goat) or conjugated to horseradish peroxidase (Anti-Rabbit and Anti-mouse IgG (H+L), 1:8.000, Vector Laboratories).
Ola R., Künzel S.H., Zhang F., Genet G., Chakraborty R., Pibouin-Fragner L., Martin K., Sessa W., Dubrac A, & Eichmann A. (2018). SMAD4 prevents flow induced arterial-venous malformations by inhibiting Casein Kinase 2. Circulation, 138(21), 2379-2394.
+ Open protocol
+ Expand
4

Flow Cytometric Analysis of Notch Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were washed once with cold PBS, prior to dissociation with Versene (Life Technologies), washed twice more in PBS / 0.1% Fetal calf serum, blocked in 1% mouse or rabbit serum before incubation with combinations of the following fluorochrome-conjugated antibodies: anti-NOTCH1 (Ebioscience, 17-9889, 1:50); anti-JAGGED1 (R&D systems, FAB1726A, 1:8); anti-latency associated peptide (Ebioscience, 17-9829, 1:20). Cells were then washed twice more, before fixation with 4% PFA and analysis on a FACSCalibur (Becton Dickenson). Flow-based cell sorting was conducted on a FACSAria II cytometer (Becton Dickenson). Flow data was analysed with FlowJo v10.
Hoare M., Ito Y., Kang T.W., Weekes M.P., Matheson N.J., Patten D.A., Shetty S., Parry A.J., Menon S., Salama R., Antrobus R., Tomimatsu K., Howat W., Lehner P.J., Zender L, & Narita M. (2016). NOTCH1 mediates a switch between two distinct secretomes during senescence. Nature cell biology, 18(9), 979-992.
+ Open protocol
+ Expand
5

Immunohistochemical Analysis of Kidney Development

Check if the same lab product or an alternative is used in the 5 most similar protocols
anti-jagged1 (R&D Systems, Minneapolis, MN), anti-E-cadherin (BD Transduction Laboratories, UK), anti-wt1 (Santa Cruz, Dallas, TX), anti-LEF1 (Cell Signaling, The Netherlands), anti-Pax8 (Proteintech Europe, UK), anti-Pax2 (Covance, Princeton, NJ), anti-phospho-β-cat (S33, S37, T41) (Cell Signaling), anti-phospho-β-cat (S45) (Cell Signaling), pan β-cat (SIGMA), anti-dephospho-β-cat (AG Scientific, San Diego, CA), anti-podocalyxin, anti-laminin (SIGMA), LTL (Vector Labs, Burlingame, CA), anti-ZO1 (DSHB, Iowa City, IA), anti-pSMAD (Cell Signaling), anti-pAKT (Cell Signaling), 6-CF (SIGMA), PNA (Vector Labs), Annexin V (Biovision, San Francisco, CA), TUNEL (ROCHE, Switzerland), anti-phospho-β-cat (S552) (Cell Signaling).
Lindström N.O., Lawrence M.L., Burn S.F., Johansson J.A., Bakker E.R., Ridgway R.A., Chang C.H., Karolak M.J., Oxburgh L., Headon D.J., Sansom O.J., Smits R., Davies J.A, & Hohenstein P. (2015). Integrated β-catenin, BMP, PTEN, and Notch signalling patterns the nephron. eLife, 4, e04000.
+ Open protocol
+ Expand
6

Multiparametric Analysis of Notch Signaling in Leukemia

Check if the same lab product or an alternative is used in the 5 most similar protocols
The antibodies used for FACS analysis were: mouse IgG2b-FITC, goat IgG-PE, anti-Jagged1-FITC, anti-Dll3-PE (all from R&D System, Minneapolis, MN), mouse IgG2a-PE, mouse IgG1κ-PE, mouse IgG1-Alexa Fluor 488,anti-Notch1-PE, anti-Notch2-PE, anti-Notch3-PE, anti-Notch4-PE, anti-Dll1-PE, anti-Dll4, anti-Bax-Alexa Fluor 488 (all from Biolegend, San Diego, CA) and rabbit anti-Bcl-2-FITC (DAKO). For leukemia cell identification we used anti-CD45-VioBlue, anti-CD45-APC-Vio770, anti-CD34-PerCP and anti-CD117-APC (all from MiltenyiBiotec, Germany). The antibodies employed for western blot analysis anti-Notch2, anti-Notch4 were from Santa Cruz (Biotechnology, Dallas, TX), anti-GAPDH and HRP conjugated secondary antibodies against mouse, rabbit or goat were from Sigma Aldrich. All the other antibodies used for Western blot were from Cell Signalling. Neutralizing antibodies,all used at a final concentration of 5 μg/ml, were: anti-Notch1, anti-Notch3,anti-Jagged1, anti-Jagged2, anti-Dll1 and anti-Dll4 (R&D Systems); anti-Notch-4 (Santa Cruz Biotechnology); anti-Dll3 (CST, Boston, MA). Recombinant human Jagged-1 and Jagged-2 were from R&D System. GSI-IX (DAPT) was purchased from Stemgent (Cambridge, MA) GSI-XII and SAHM1 were from Merck Millipore (Darmstadt, Germany). Cytarabine (Ara-C), Etoposide (Eto) and Idarubicin (Ida) were provided by Pharmacy Unit of the University Hospital of Verona.
Kamga P.T., Bassi G., Cassaro A., Midolo M., Di Trapani M., Gatti A., Carusone R., Resci F., Perbellini O., Gottardi M., Bonifacio M., Kamdje A.H., Ambrosetti A, & Krampera M. (2016). Notch signalling drives bone marrow stromal cell-mediated chemoresistance in acute myeloid leukemia. Oncotarget, 7(16), 21713-21727.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!