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Irdye conjugate secondary antibodies

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IRDye conjugate secondary antibodies are fluorescent-labeled antibodies that can be used to detect and quantify target proteins in various immunoassays, such as Western blotting, immunohistochemistry, and flow cytometry. These antibodies are conjugated with near-infrared dyes, which offer improved signal-to-noise ratio and deeper tissue penetration compared to visible fluorescent dyes.

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Lab products found in correlation

Alexa flour secondary antibody, by Thermo Fisher Scientific (1 mentions) Alexa fluor secondary antibody, by Thermo Fisher Scientific (1 mentions) Goat anti fibrinogen, by Merck Group (1 mentions)

Close spelling variants of this product

IRDye antibodies (21 citations) IRDye conjugates (3 citations)

2 protocols using irdye conjugate secondary antibodies

1

Multicolor Immunofluorescence Labeling

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Alexa Flour 488–labeled donkey anti-goat, Alexa Flour 594–labeled donkey anti-mouse, Alexa Flour 647–labeled donkey anti-rabbit, IRDye 800CW donkey anti-goat, and IRDye 680LT goat anti-rabbit were used. Alexa Flour secondary antibodies were purchased from Invitrogen Molecular Probes, and IRDye conjugate secondary antibodies were from LI-COR Biosciences.
Flores-Mireles A.L., Pinkner J.S., Caparon M.G, & Hultgren S.J. (2014). EbpA vaccine antibodies block binding of Enterococcus faecalis to fibrinogen to prevent catheter-associated bladder infection in mice. Science translational medicine, 6(254), 254ra127.
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2

Immunofluorescent Detection of Bacterial Pathogens

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Primary antibodies: goat anti-fibrinogen (Sigma-Aldrich), mouse anti-EbpABC (1:1000) for E. faecalis [43 (link)] or rabbit anti-E.coli serotype O/K (Invitrogen) (1:100) for P. mirabilis [60 (link)]. Secondary antibodies: Alexa Fluor 488-labeled donkey anti-mouse, Alexa Fluor 594-labeled donkey anti-rabbit, Alexa Fluor 647-labeled donkey anti-goat, donkey anti-goat IRDye 800CW, donkey anti-goat IRDye 680LT, donkey anti-rabbit IRDye 680LT, or donkey anti-mouse IRDye 800CW were used. Alexa Fluor secondary antibodies were purchased from Invitrogen Molecular Probes, and IRDye conjugate secondary antibodies were from LI-COR Biosciences.
Gaston J.R., Andersen M.J., Johnson A.O., Bair K.L., Sullivan C.M., Guterman L.B., White A.N., Brauer A.L., Learman B.S., Flores-Mireles A.L, & Armbruster C.E. (2020). Enterococcus faecalis Polymicrobial Interactions Facilitate Biofilm Formation, Antibiotic Recalcitrance, and Persistent Colonization of the Catheterized Urinary Tract. Pathogens, 9(10), 835.
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