Plan apochromat 10x 0.45 na 2 mm working

Imaging was conducted on a motorized Axioskop 2 FS plus microscope (Zeiss) using a Plan-Apochromat 10X 0.45 NA 2 mm working distance objective lens (1063–139, Zeiss). Imaging plates were controlled by a motorized stage (ProScan II, Prior) with a stage-mounted customized Petri dish holder. Images were acquired with Metamorph 7.5.3.0 software. All microscopy was performed at 22°C. Excitation at 410 nm and 470 nm was performed sequentially with a high-speed filter changer (Lambda DG-4, Shutter Instrument Company) controlling the excitation filters D410/30x and D470/20x. The filtered light was reflected by a 500dcxr dichroic mirror to illuminate the specimen. The emitted fluorescence passed through the filter HQ535/50m and was detected with a Cool SNAP HQ^{2 (link)} 14-bit camera (Photometrics). All filters were manufactured by Chroma.