Hdac7

Manufactured by Proteintech

Sourced in United States

HDAC7 is a histone deacetylase enzyme that plays a role in the regulation of gene expression. It is responsible for the removal of acetyl groups from lysine residues on histone proteins, which can lead to changes in chromatin structure and transcriptional activity.

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2 protocols using hdac7

Protein Extraction and Western Blot Analysis

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We extracted protein from cells and tissues, and we quantified the concentration using the BCA protein detection kit (Beyotime, China). Separated proteins were transferred to polyvinylidene fluoride (PVDF) membranes by adding 10% or 12% SDS‒PAGE. The PVDF membrane was incubated with the primary antibody overnight at 4 °C, followed by 1 h of incubation with horseradish peroxidase-conjugated secondary antibodies. Target proteins were detected using an enhanced chemiluminescence system. Images were acquired using a multifunctional UVP ChemStudio/PLUS imager (Jena, Thuringia, Germany). The antibodies used were as follows: AZGP1, HDAC1, H3K27Ac from Abcam (Abcam, Cambridge, UK); smad3, p-smad3, and GAPDH from CST (CST, Beverly MA, USA); TGF-β1, E-cadherin, N-Cadherin, Vimentin, HDAC2, HDAC3, and HDAC7 from Proteintech (Proteintech, Chicago, USA).

Deng L., Bao W., Zhang B., Zhang S., Chen Z., Zhu X., He B., Wu L., Chen X., Deng T., Chen B., Yu Z., Wang Y, & Chen G. (2023). AZGP1 activation by lenvatinib suppresses intrahepatic cholangiocarcinoma epithelial-mesenchymal transition through the TGF-β1/Smad3 pathway. Cell Death & Disease, 14(9), 590.

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Western Blot Characterization of Signaling Pathways

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Western blotting was performed according to the procedures described previously [20 (link)], loading 25 μg of total protein lysate per lane. Primary antibodies were diluted at 1:1000 and secondary antibodies were diluted at 1:5000. Antibodies against the following proteins were used for the study: HDAC7 (#33418), USP10 (#8501), β-catenin (#8480), acetyl-β-catenin (Lys49; #9030), phospho-β-catenin (Thr41/Ser45; #9565), phospho-β-catenin (Ser552; #5651), phospho-β-catenin (Ser675; #4176), Cyclin E1 (#4129), E-cadherin (#14472), Slug (#9585), β-actin (#3700), Lamin B1 (#13435), and GAPDH (#5174) (CST). HDAC7(26207–1-AP), CDK1(19532–1-AP), CDK2 (10122–1-AP), CDK4 (11026–1-AP), CDK6 (14052–1-AP), Cyclin A2 (66391–1-Ig), Cyclin B1 (55004–1-AP), Cyclin D1 (60186–1-Ig), Cyclin D3 (26755–1-AP), FGF18 (60341–1-Ig), FGFR3 (66954–1-Ig), Snail (13099–1-AP), vimentin (10366–1-AP), ZEB1 (21544–1-AP), Flag (66008–3-Ig), and ubiquitin (10201–2-AP) were also used (Proteintech Group).

Guo K., Ma Z., Zhang Y., Han L., Shao C., Feng Y., Gao F., Di S., Zhang Z., Zhang J., Tabbò F., Ekman S., Suda K., Cappuzzo F., Han J., Li X, & Yan X. (2022). HDAC7 promotes NSCLC proliferation and metastasis via stabilization by deubiquitinase USP10 and activation of β-catenin-FGF18 pathway. Journal of Experimental & Clinical Cancer Research : CR, 41, 91.

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