Fetal bovine serum antibiotic solution

Roswell Park Memorial Institute 1640 (RPMI-1640) medium, fetal bovine serum (FBS) antibiotic solution (penicillin 1000 IU and streptomycin 10 mg/mL), and MTT (3-(4,5-dimethylthiazol-2-yl)-2, 5 diphenyltetrazolium bromide dye) were purchased from [Himedia, India], whereas DMSO (dimethyl sulphoxide), RNAse, and proteinase K were obtained from GeNie, Merck, India. Ethidium bromide, Triton X-100, and other chemicals of analytical grades were purchased from Lobachemie Pvt. Ltd., India. Acridine orange was obtained from SRL Pvt. Ltd., India, SDS (sodium dodecyl sulphate) was obtained from Bio basic Inc., India, and agarose was purchased from Sigma, USA, whereas propidium iodide (PI) was obtained from EMD Millipore-Calbiochem, USA.

Trypan blue, bovine serum albumine (BSA) and gelatin were purchased from Sigma-Aldrich, USA. Olaparib and 3, 4-dihydro-5-[4-(1-piperidinyl) butoxy]-1(2H)-isoquinolinone (DPQ) from Selleckchem, UK. Trypsin, fetal bovine serum (FBS), antibiotic solution and DMEM were purchased from HiMedia, India. The detail information about antibodies used is given in Additional file 1. Other reagents and bio-chemicals were purchased locally.