The CD4+ and CD8+ T lymphocyte counts were performed by flow cytometry (BD FACScaliburTM, Becton and Dickinson, San Jose, CA, USA) using the FACScountTM Reagents monitoring kit, following the protocol recommended by the manufacturer (Becton and Dickinson, San Jose, CA, USA). The results of the lymphocytes are expressed in cells/µL.
Facscount reagents monitoring kit
Manufactured by BD
Sourced in United States
The FACScountTM Reagents monitoring kit is a laboratory equipment designed to assist in the monitoring and analysis of reagents used in flow cytometry procedures. The core function of this product is to provide accurate and reliable data on the status and performance of the reagents, enabling researchers and laboratory professionals to maintain the integrity of their flow cytometry workflows.
Automatically generated - may contain errors
4 protocols using facscount reagents monitoring kit
1
Flow Cytometric T Cell Enumeration
2
T Cell Quantification and Viral Load Measurement
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CD4+ TL and CD8+ TLs were quantified by flow cytometry (BD FACSCaliburTM, Becton & Dickinson, Franklin Lakes, NJ, USA) with the FACSCountTM Reagents monitoring kit, following the protocol recommended by the manufacturer (Becton & Dickinson, Franklin Lakes, NJ, USA). The viral load was quantified by real-time PCR using the Sample Purific CV HIV-1 extraction kit (Abbott, Chicago, IL, USA) and the HIV-1 viral load amplification kit (Abbott, Chicago, IL, USA). The units used were copies/mL and log10. The determinations followed the standard established by the National Network for the Determination of CD4+ and CD8+ T cells and Viral Load of the Department of HIV/AIDS and Viral Hepatitis of the Brazilian Ministry of Health. A statistical evaluation of the plasma levels of CD4+ and CD8+ TL was performed on the median values of each group.
3
Flow Cytometry CD4+ and CD8+ Enumeration
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The CD4+ and CD8+ T cells were counted by flow cytometry (BD FACSCalibur™, Becton & Dickinson) with the FACSCount™ Reagents monitoring kit, following the protocol recommended by the manufacturer (Becton & Dickinson, San Jose, California, USA).
4
T Cell Enumeration by Flow Cytometry
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The CD4 + and CD8 + T cells were counted by ow cytometry (BD FACSCalibur™, Becton & Dickinson) with the FACSCount™ Reagents monitoring kit, following the protocol recommended by the manufacturer (Becton & Dickinson, San Jose, California, USA).
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