Vacutainer sst 2 advance plus blood collection tube

After 14 weeks of Wi-Fi exposure, each animal was euthanized with a Ketamine-Tiletamine-Xylazine (KTX) cocktail intraperitoneally. Blood was drawn immediately from retro-orbital sinus and collected in BD Vacutainer SST II Advance Plus Blood Collection Tube (BD, United States). The blood was left undisturbed for at least 30 min at room temperature, and the collection tubes were centrifuged at 1,500 g for 10 min at 4°C. The obtained serum was aliquoted and stored at −80°C until analysis.
Both sides of the testis, epididymis and seminal vesicle were carefully dissected and cleaned from surrounding adipose tissue before weighing. The organ coefficient of each dissected reproductive organ was expressed according to the equation: the wet weight of organ (g)/body weight (g) × 100 (Feng et al., 2015 (link)). The right testis was fixed in 10% buffered neutral formalin (Merck, Germany), processed with standard protocol, and embedded in paraffin wax (Merck, Germany). The left testis was immediately snap-frozen and stored at −80°C until analysis.

Blood was drawn immediately from retro-orbital sinus following sacrifice. Blood was collected in BD Vacutainer SST II Advance Plus Blood Collection Tube (BD, United States) and left undisturbed for at least 30 min at room temperature. The collection tubes were then transferred into the centrifuge and serum was separated by centrifugation at 1500 g for 10 min at 4°C. Serum yields were aliquoted and stored at −80°C until analysis.

Whole blood of patients suspected of lung carcinoma was collected in an 8.5 mL BD Vacutainer SSTII Advance Plus Blood Collection Tube during routine venipuncture. The tubes were processed within one hour after collection by centrifugation for 10 minutes at 2683 g at 20°C. Serum was aliquoted in 2 mL VWR microtubes and stored at –80°C until further analysis.
Pure hemolysate pools were prepared by collecting heparinized whole blood samples of 10 healthy volunteers. Blood of one volunteer (pool f) was collected at three time points, day 1 = time point 0, day 2 = 3 weeks after day 1, day 3 = 4.5 months after day 1. The tubes were centrifuged for 5 minutes at 2000 g and the plasma was removed. To remove any extracellular NSE, the remaining cells were washed three times by adding an equal volume of physiological salt. The cells were then dissolved in physiological saline and lysed by freezing them for at least 2 hours at –80°C. After thawing and centrifugation for 5 min at 2000 g, the cellular debris was removed and dilutions of 0%, 1%, 2%, 2.5%, 3%, 4%, 5%, 6%, 10%, 20%, 30% and 40% hemolysate in physiological saline were prepared.