Dulbecco's modified Eagle's medium/nutrient mixture F-12 (DMEM-F12), penicillin/streptomycin and fetal bovine serum (FBS) were obtained from Gibco. Collagenase P were obtained from Roche. Anti-human CD34-PE, CD45-FITC, HLA-DR-FITC, CD44-FITC, CD73-PE, CD90-FITC, and were purchased from BD Biosciences. Adipogenic and osteogenic inducing medium from Cyagen. Matrigel were obtained from Corning. Trizol Reagent were purchased by Invitrogen. All-In-One RT MasterMix Kit, EvaGreen qPCR MasterMix Kit were obtained from abmGood. Cell-tracker dye CM-DiI were purchased from Invitrogen.
All in one rt mastermix kit
Manufactured by Abmgood
Sourced in Canada
The All-In-One RT MasterMix Kit is a laboratory equipment product designed for reverse transcription and real-time PCR. It contains all the necessary components for cDNA synthesis and gene expression analysis in a single solution.
Automatically generated - may contain errors
Lab products found in correlation
Trizol reagent, by Thermo Fisher Scientific (2 mentions)
Evagreen qpcr mastermix kit, by Abmgood (2 mentions)
Dulbecco s modified eagle s medium nutrient mixture f 12 dmem f 12, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Cd45 fitc, by BD (1 mentions)
Cd73 pe, by BD (1 mentions)
Hla dr fitc, by BD (1 mentions)
Cd44 fitc, by BD (1 mentions)
Cd90 fitc, by BD (1 mentions)
Anti human cd34 pe, by BD (1 mentions)
Matrigel, by Corning (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Collagenase p, by Roche (1 mentions)
Cell tracker dye cm dii, by Thermo Fisher Scientific (1 mentions)
Taq pro universal sybr qpcr master mix, by Vazyme (1 mentions)
Accurt genomic dna removal kit, by Abmgood (1 mentions)
3 protocols using all in one rt mastermix kit
1
Isolation and Characterization of Mesenchymal Stem Cells
2
Liver and Gut Tissue Gene Expression
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An appropriate amount of liver and hindgut tissue was thoroughly ground in the presence of liquid nitrogen. Total RNA was extracted following the instructions of the EASYspin plus kit (Beijing Adlai Biotechnology Co., Ltd., China). First-strand cDNA was synthesized using a 5X All-In-One RT MasterMix Kit (with AccuRT Genomic DNA Removal Kit, abmGood) and stored at -20°C. The mRNA expression of fas, cpt1, acc, nfkb, il1b, and tnfa, whose primer sequences are shown in Table 2 , was determined by a relative quantitative method following the instructions of Taq Pro Universal SYBR qPCR Master Mix (Vazyme, Nanjing, China). β-Actin was used as an internal reference gene.
3
Quantifying Gene Expression in hDPSCs
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Cultured hDPSCs in P4 were collected and total RNA were extracted with Trizol Reagent (Invitrogen). cDNA was synthesized from RNA using All-In-One RT MasterMix Kit (abmGood, Canada). qPCR was performed using EvaGreen qPCR MasterMix Kit (abmGood, Canada). Thermal cycling conditions were 95°C for 10 min and 40 cycles of 95°C for 15 s, 60°C for 60 s. The sequences of primers were listed in Table 1
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