The slitted tubular struts were fabricated using an Objet 3D printer (Objet 260 Connex, StrataSys Inc, Eden Prairie, MN, USA) in digital material mode using the PolyJet technology. The printer can combine two base materials, using pre-determined ratios to make the so-called digital materials. The digital materials differ in mechanical and thermal properties. The curable liquid photopolymer was jetted onto the build tray and then cured by UV polymerization. The three digital materials used in this paper are Verowhite plus, DM9895 (DM-1) and DM8530 (DM-2) in Stratasys material library. The cables were fabricated using the Fused Filament Fabrication (FFF) technology on a HYREL 3D Printer (System 30 M, Hyrel 3D Inc, Norcross, GA, USA). A rubbery material named Filaflex (Recreus, Elda, Spain) was used, which is a thermoplastic elastomer base polyurethane. The extruder was especially equipped with a dual drive system to fulfill the task of printing flexible filaments. The filament was melted at ~232 °C and deposited through a nozzle of 500 µm diameter onto the tray. The cable nets were printed by two passes of reversed orientation. The extrusion paths were optimized to ensure the quality of the printing.
Objet260 connex
Manufactured by Stratasys
Sourced in United States, Mongolia
The Objet260 Connex is a 3D printer designed for professional use. It offers multi-material printing capabilities, allowing for the creation of complex, multi-color, and multi-texture parts. The Objet260 Connex is capable of producing high-quality 3D models and prototypes.
Automatically generated - may contain errors
Lab products found in correlation
Dexamethasone (dex), by MP Biomedicals (2 mentions)
Dmem high glucose, by Merck Group (2 mentions)
L ascorbic acid 2 phosphate, by Fujifilm (2 mentions)
Sodium pyruvate hyclone, by Thermo Fisher Scientific (2 mentions)
Non essential amino acids (neaa), by Lonza (2 mentions)
Sylgard 184, by Dow (2 mentions)
Its premix, by Corning (2 mentions)
Med610, by Stratasys (2 mentions)
Uprint se, by Stratasys (1 mentions)
Autocad, by Autodesk (1 mentions)
9 protocols using objet260 connex
1
Additive Manufacturing of Hybrid Bioinspired Structures
2
Annular 3D-Printed Cell Culture
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Custom annular culture well molds (2 mm diameter posts; 3.75 mm wide trough) were 3D printed (Objet260 Connex; Stratasys, Eden Prairie, MN). Polydimethylsiloxane (PDMS; Sylgard 184, Dow Corning, Midland, MI) was cured in the printed molds and served as a negative for casting the 2% w/v agarose (Denville Scientific Inc., Metuchen, NJ) culture wells used in this study. Prior to cell seeding, culture wells were incubated over night in serum-free, chemically defined basal medium comprised of high-glucose DMEM (Sigma-Aldrich) with 10% ITS+ Premix (Corning; Fisher Scientific), 1 mM sodium pyruvate (HyClone; Fisher Scientific), 100 μM non-essential amino acids (Lonza, Basel, Switzerland), 100 nM dexamethasone (MP Biomedicals, Solon, OH), 0.12 mM L-ascorbic acid-2-phosphate (Wako), and 1% P/S (Fisher Scientific) as described previously 30 (link).
3
Digital Implant Planning and Surgical Guide Fabrication
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The resulting alignment was visually examined by a technician to check the correctness and repeat the procedure if necessary. Once the alignment was verified, the surgeon could then perform a virtual implant treatment plan with the software. Considering the ideal prosthetic and anatomic conditions, an implant with proper diameter and length was virtually planned with the implant planning software. The digital implant treatment planning was then exported in STL format to computer-aided design (CAD) software (PlastyCAD, 3DIEMME Bioimaging Tecnologies, Figino Serenza, Italy), and the surgical guide was designed according to this planned implant position. The completed CAD files of the surgical guide were then uploaded to the computer-aided manufacturing (CAM) software (GrabCAD Print, Stratasys Ltd., Eden Prairie, MN, USA) of a 3D printer (Objet260 Connex, Stratasys Ltd.), and an SLA surgical guide (BenQ AB Guide, BenQ AB DentCare Corp., Taipei, Taiwan) was fabricated by a certificated manufacturer using the modeling resin material (MED610, Stratasys Ltd.) with the following settings: 2 mm of thickness and 0.08 mm of guide-to-teeth offset. Metal sleeves (4.5 mm in height and 5.0 mm in diameter) specific to the surgical guide system were inserted into the surgical guide. All the surgical guides were tooth-supported. Figure 3 illustrated the workflow of the digital implant planning.
4
Personalized 3D-Printed IANB Device
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The creation and design of the IANB device followed the procedures described previously [6 ]. The dentition of each participant was optically scanned using TRIOS 3 (3 shape, Poland), and the obtained dentition data were matched with existing CT data of the mandible using computer-aided design (CAD) software (Materialize Magics™, Materialize, Leuven, Belgium). The IANB device was designed and printed with a 3D printer (Objet 260 connex™, Stratasys, Eden Prairie, USA). Biocompatible resin MED610 (Stratasys, Eden Prairie, USA) and supporting material SUP705B (Polymerized™, Stratasys, Eden Prairie, USA) were used. As part of the design, a safety margin was created by providing a guide and stopper with a target point 5 mm in front of the mandibular lingula (Figure 1 ). If the IANB device was not placed in the correct position, the intraoral scanning process was repeated to create the device again.
5
Characterization of 3D Printed Polymer Lattices
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The specimens used in our experiments are rigid polymer (VeroWhite) lattice structures prepared by 3D printing from designed CAD models by using an Objet260 Connex 3D printer (Stratasys Ltd., USA). The geometric parameters of the tetragonal lattice are θ = 77°, l = 12 mm, b = 0.3 mm, and h = 1.2 mm, while those of the hexagonal lattice are θ = 55°, l = 12 mm, b = 0.3 mm, and h = 1.2 mm. Each specimen has two much thicker and stiffer frames on two opposite sides, so that uniform displacements can be applied to the boundary in tension tests. The frames are then cut in bending tests. Tension test is conducted via In Situ Microscope with Microtest (JP-Nikon TiS; GB-Microtest 5000W). Transverse contraction means positive IPR while transverse expansion indicates negative IPR of the specimen. As uniform bending with constant moment is hard to realize, we bend a specimen with hands. The BPR is positive, zero, and negative if the specimen shows a saddle, cylinder, and dome shape.
6
3D-Printed Annular Culture Wells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Custom annular culture well molds (2 mm diameter posts; 3.75 mm wide trough) were 3D printed (Objet260 Connex; Stratasys, Eden Prairie, MN). Polydimethylsiloxane (PDMS; Sylgard 184, Dow Corning, Midland, MI) was cured in the printed molds and served as a negative for casting 2% w/v agarose culture wells (Denville Scientific Inc., Metuchen, NJ). Prior to cell seeding, culture wells were incubated over-night in serum-free, chemically defined basal medium comprised of high-glucose DMEM (Sigma-Aldrich) with 1% ITS+ Premix (Corning; Fisher Scientific), 1 mM sodium pyruvate (HyClone; Fisher Scientific), 100 μM non-essential amino acids (Lonza, Basel, Switzerland), 100 nM dexamethasone (MP Biomedicals, Solon, OH), 0.13 mM l -ascorbic acid-2-phosphate (Wako), and 1% P/S (Fisher Scientific)29 (link)–31 (link).
7
3D Printing of VeroWhite Resin Abutments
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The STL file of the completed design was printed using a Stratasys Objet260 Connex 3D printer. VeroWhite resin was used as the material. The weight of the resin abutment was 14 g, the thickness of the printed layer was 0.03 mm, and a total of 30 pieces were printed (Fig 1 ).
8
Fabrication of Microfluidic Devices
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All devices shown in Fig. 1234and Video S1-S8 † were fabricated using a Stratasys Polyjet printer (Objet 260 Connex) with Vero+ resin. Each device had overall dimensions of 12 cm × 3.8 cm × 0.8 cm, with two screw holes at either end to rigidly fasten the device to the support platform. Channels within the device were typically 2 mm in width and 5 mm deep. Circular entry or exit reservoirs typically had a raised edge that protruded 2 mm above the nominal top surface of the device to help prevent spillage.
For some experiments, it was convenient to use a black device to maximize visual contrast with a white powder. These black devices were printed using a Formlabs Form 2 SLA printer with BioMed Black Formlabs resin, using identical geometry as the white devices.
For some experiments, it was convenient to use a black device to maximize visual contrast with a white powder. These black devices were printed using a Formlabs Form 2 SLA printer with BioMed Black Formlabs resin, using identical geometry as the white devices.
9
Microdrive Design and 3D Printing
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All 3D printed devices and assemblies described in this manuscript were designed with AutoCAD (Autodesk).
Microdrive bodies were printed with an Objet260 Connex (Stratasys) printer with Vero PolyJet material. Drive screw holes and flexible drive axis (FDA) holes were cleared using size #71 and/or #64 drill bits in preparation for thread tapping.
The headmount cap and base, and microdrive placement tool, were printed using a uPrint SE (Stratasys) 3D printer.
Microdrive bodies were printed with an Objet260 Connex (Stratasys) printer with Vero PolyJet material. Drive screw holes and flexible drive axis (FDA) holes were cleared using size #71 and/or #64 drill bits in preparation for thread tapping.
The headmount cap and base, and microdrive placement tool, were printed using a uPrint SE (Stratasys) 3D printer.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!