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Microfabricated silicon cantilevers

Manufactured by Nanosensors
Sourced in United States

Microfabricated silicon cantilevers are small, precision-engineered devices designed for use in a variety of scientific and technological applications. They consist of a thin, flexible arm made of silicon that can detect and measure minute changes in physical, chemical, or biological parameters. The core function of these cantilevers is to serve as sensitive transducers, converting various stimuli into measurable signals.

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3 protocols using microfabricated silicon cantilevers

1

Atomic Force Microscopy Imaging of Lysozyme Fibrils

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For the AFM imaging
study, the solutions
of lyz fibrils were diluted to 100-fold with water. Then, around 5
μL of this sample solution was adsorbed onto a freshly cleaved
muscovite ruby mica sheet (ASTM VI grade Ruby Mica from Micafab, Chennai,
India). Thereafter, the mica sheet was dried for 30 min in vacuum
in an inert atmosphere. The complexes were incubated for 15 min prior
to adsorption onto the mica sheet. AFM was performed in the AAC mode
on PicoPlus 5500 ILM AFM (Agilent Technologies, USA), which was attached
with a piezo-scanner of a maximum range of 9 μm. Here, microfabricated
silicon cantilevers of NANOSENSORS (USA) were used. The resonance
frequency of the cantilever oscillation was 146–236 kHz, whereas
the force constant was 21–98 N/m. The rate of the scan speed
was 0.5 lines/s while taking the images (256 by 256 pixels). All the
images were processed by flattening using PicoView software (Agilent
Technologies, 1.1 version), whereas their manipulation was conducted
by Pico Image Advanced version software.
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2

Atomic Force Microscopy of Extracellular Vesicles

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For AFM imaging, purified EVs preparations were diluted with filtered PBS (1:100) and a 5 μl aliquot of the diluted sample solution was deposited on freshly cleaved mica sheet followed by incubation at room temperature for 10 min. The dried sample was then gently washed with 200 μl of Milli-Q water to remove salt and loosely bound moieties. AFM experiments were performed in intermittent contact mode (called “tapping” or AAC mode) to minimize tip-induced damage. AAC mode AFM was performed using a Pico plus 5500 inverted light microscope AFM (Agilent Technologies) with a Piezo scanner (maximum range 9 μm). Microfabricated silicon cantilevers 225 μm in length with a nominal spring force constant of 21 to 98 N/m were used (Nano Sensors). Cantilever oscillation frequency was tuned into resonance frequency. The cantilever resonance frequency was 150 to 300 kHz. The images (512 × 512 pixels) were captured with a scan size between 0.5 and 0.8 μm at a scan speed rate of 0.5 lines/s. Images were processed by flatten using Pico view1.1 version software (Agilent Technologies). Image manipulation was done using Pico Image Advanced version software (Agilent Technologies).
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3

Atomic Force Microscopy of Biomolecules

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We primarily followed the procedure described previously (Henn et al., 2001 (link)). AFM experiments were performed in intermittent contact mode (called “tapping” or AAC mode) to minimize tip-induced damage. AAC mode AFM was performed using a Pico plus 5500 inverted light microscope AFM (Agilent Technologies) with a Piezo scanner (maximum range 9 µm). Microfabricated silicon cantilevers 225 µm in length with a nominal spring force constant of 21–98 N/m were used (Nano Sensors). Cantilever oscillation frequency was tuned into resonance frequency. The cantilever resonance frequency was 150–300 kHz. The images (512 × 512 pixels) were captured with a scan size between 0.5 and 0.8 µm at a scan speed rate of 0.5 lines/s. Images were processed by flatten using Pico view1.1 version software (Agilent Technologies). Image manipulation was done using Pico Image Advanced version software (Agilent Technologies).
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