Microfabricated silicon cantilevers

Manufactured by Nanosensors

Sourced in United States

Microfabricated silicon cantilevers are small, precision-engineered devices designed for use in a variety of scientific and technological applications. They consist of a thin, flexible arm made of silicon that can detect and measure minute changes in physical, chemical, or biological parameters. The core function of these cantilevers is to serve as sensitive transducers, converting various stimuli into measurable signals.

Automatically generated - may contain errors

Lab products found in correlation

Pico plus 5500 inverted light microscope afm, by Agilent Technologies (2 mentions) Pico view1.1 version software, by Agilent Technologies (2 mentions) Pico image advanced version software, by Agilent Technologies (2 mentions) Picoview software, by Agilent Technologies (1 mentions) Pico plus 5500 ilm afm, by Agilent Technologies (1 mentions)

Close spelling variants of this product

Silicon cantilevers

3 protocols using microfabricated silicon cantilevers

Atomic Force Microscopy Imaging of Lysozyme Fibrils

Check if the same lab product or an alternative is used in the 5 most similar protocols

For the AFM imaging
study, the solutions
of lyz fibrils were diluted to 100-fold with water. Then, around 5
μL of this sample solution was adsorbed onto a freshly cleaved
muscovite ruby mica sheet (ASTM VI grade Ruby Mica from Micafab, Chennai,
India). Thereafter, the mica sheet was dried for 30 min in vacuum
in an inert atmosphere. The complexes were incubated for 15 min prior
to adsorption onto the mica sheet. AFM was performed in the AAC mode
on PicoPlus 5500 ILM AFM (Agilent Technologies, USA), which was attached
with a piezo-scanner of a maximum range of 9 μm. Here, microfabricated
silicon cantilevers of NANOSENSORS (USA) were used. The resonance
frequency of the cantilever oscillation was 146–236 kHz, whereas
the force constant was 21–98 N/m. The rate of the scan speed
was 0.5 lines/s while taking the images (256 by 256 pixels). All the
images were processed by flattening using PicoView software (Agilent
Technologies, 1.1 version), whereas their manipulation was conducted
by Pico Image Advanced version software.

Saha B., Chowdhury S., Sanyal D., Chattopadhyay K, & Suresh Kumar G. (2018). Comparative Study of Toluidine Blue O and Methylene Blue Binding to Lysozyme and Their Inhibitory Effects on Protein Aggregation. ACS Omega, 3(3), 2588-2601.

+ Open protocol

+ Expand

Atomic Force Microscopy of Extracellular Vesicles

Check if the same lab product or an alternative is used in the 5 most similar protocols

For AFM imaging, purified EVs preparations were diluted with filtered PBS (1:100) and a 5 μl aliquot of the diluted sample solution was deposited on freshly cleaved mica sheet followed by incubation at room temperature for 10 min. The dried sample was then gently washed with 200 μl of Milli-Q water to remove salt and loosely bound moieties. AFM experiments were performed in intermittent contact mode (called “tapping” or AAC mode) to minimize tip-induced damage. AAC mode AFM was performed using a Pico plus 5500 inverted light microscope AFM (Agilent Technologies) with a Piezo scanner (maximum range 9 μm). Microfabricated silicon cantilevers 225 μm in length with a nominal spring force constant of 21 to 98 N/m were used (Nano Sensors). Cantilever oscillation frequency was tuned into resonance frequency. The cantilever resonance frequency was 150 to 300 kHz. The images (512 × 512 pixels) were captured with a scan size between 0.5 and 0.8 μm at a scan speed rate of 0.5 lines/s. Images were processed by flatten using Pico view1.1 version software (Agilent Technologies). Image manipulation was done using Pico Image Advanced version software (Agilent Technologies).

Das P., Mukherjee A, & Adak S. (2021). Glyceraldehyde-3-phosphate dehydrogenase present in extracellular vesicles from Leishmania major suppresses host TNF-alpha expression. The Journal of Biological Chemistry, 297(4), 101198.

+ Open protocol

+ Expand

Atomic Force Microscopy of Biomolecules

Check if the same lab product or an alternative is used in the 5 most similar protocols

We primarily followed the procedure described previously (Henn et al., 2001 (link)). AFM experiments were performed in intermittent contact mode (called “tapping” or AAC mode) to minimize tip-induced damage. AAC mode AFM was performed using a Pico plus 5500 inverted light microscope AFM (Agilent Technologies) with a Piezo scanner (maximum range 9 µm). Microfabricated silicon cantilevers 225 µm in length with a nominal spring force constant of 21–98 N/m were used (Nano Sensors). Cantilever oscillation frequency was tuned into resonance frequency. The cantilever resonance frequency was 150–300 kHz. The images (512 × 512 pixels) were captured with a scan size between 0.5 and 0.8 µm at a scan speed rate of 0.5 lines/s. Images were processed by flatten using Pico view1.1 version software (Agilent Technologies). Image manipulation was done using Pico Image Advanced version software (Agilent Technologies).

Dey S., Biswas C, & Sengupta J. (2018). The universally conserved GTPase HflX is an RNA helicase that restores heat-damaged Escherichia coli ribosomes. The Journal of Cell Biology, 217(7), 2519-2529.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!