Raji cells (ATCC) were maintained in RPMI media (Life Technologies) with 10% fetal bovine serum (FBS, Omega Scientific) and 5 mM glutamine (Life Technologies). K562 cells (ATCC) were cultured in IMDM (ATCC) media and 10% FBS. A549 and PC3 cells (ATCC) were maintained in Kaign’s modified F-12 K media (Gibco) supplemented with 10% FBS and 5 mM glutamine. Peripheral blood macrophages (Stemcell Technologies) were thawed and plated in DMEM (ATCC) and supplemented with 5 mM glutamine and 10% FBS for at least two days and no longer than 6 days before use.
Kaign s modified f 12 k media
Manufactured by Thermo Fisher Scientific
Kaign's modified F-12 K media is a specialized cell culture medium designed to support the growth and maintenance of various cell lines. It is a modification of the original F-12 K formulation, with specific adjustments to provide an optimized environment for cell cultivation.
Automatically generated - may contain errors
Lab products found in correlation
Raji cell, by American Type Culture Collection (2 mentions)
K562 cell, by American Type Culture Collection (2 mentions)
Glutamine, by Thermo Fisher Scientific (2 mentions)
Rpmi media, by Thermo Fisher Scientific (2 mentions)
Dulbecco modified eagle medium (dmem), by American Type Culture Collection (2 mentions)
Iscove modified dulbecco media (imdm), by American Type Culture Collection (2 mentions)
A549 and pc3 cells, by American Type Culture Collection (2 mentions)
Peripheral blood macrophages, by STEMCELL (2 mentions)
2 protocols using kaign s modified f 12 k media
1
Cell Culture Protocols for Various Lines
2
Cell Culture Protocols for Various Lines
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Raji cells (ATCC) were maintained in RPMI media (Life Technologies) with 10% fetal bovine serum (FBS, Omega Scientific) and 5 mM glutamine (Life Technologies). K562 cells (ATCC) were cultured in IMDM (ATCC) media and 10% FBS. A549 and PC3 cells (ATCC) were maintained in Kaign’s modified F-12 K media (Gibco) supplemented with 10% FBS and 5 mM glutamine. Peripheral blood macrophages (Stemcell Technologies) were thawed and plated in DMEM (ATCC) and supplemented with 5 mM glutamine and 10% FBS for at least two days and no longer than 6 days before use.
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