Log In Sign up
The largest database of trusted experimental protocols

Iq tl image analysis software

Manufactured by GE Healthcare
Visit Supplier

IQ-TL is an image analysis software developed by GE Healthcare. It is designed to facilitate the processing and analysis of digital images acquired from various imaging modalities. The software provides tools for image visualization, enhancement, and quantification, enabling users to extract relevant information from the images.

Automatically generated - may contain errors

Lab products found in correlation

Nitrocellulose membrane, by Bio-Rad (2 mentions) Bradford assay, by Bio-Rad (1 mentions) Criterion xt bis tris gel, by Bio-Rad (1 mentions) Ecl prime western blotting detection substrate, by Cytiva (1 mentions) Memcode reversible protein stain kit, by Thermo Fisher Scientific (1 mentions)

3 protocols using iq tl image analysis software

1

Gelatin Zymography for Protease Activity

Check if the same lab product or an alternative is used in the 5 most similar protocols
Samples (25 μg soluble fraction total protein) were loaded onto nondenaturing 10% polyacrylamide gels containing 0.1% gelatin, electrophoresed, renatured, and developed as described previously [17 (link)]. Gel images were acquired and inverted, and densitometry was measured using the IQ-TL image analysis software (GE Healthcare, Waukesha, WI) to quantify activity.
Iyer R.P., de Castro Brás L.E., Patterson N.L., Bhowmick M., Flynn E.R., Asher M., Cannon P.L., Deleon-Pennell K.Y., Fields G.B, & Lindsey M.L. (2016). Early matrix metalloproteinase-9 inhibition post-myocardial infarction worsens cardiac dysfunction by delaying inflammation resolution. Journal of molecular and cellular cardiology, 100, 109-117.
+ Open protocol
+ Expand
2

Quantifying Protein Levels in Cellular Fractions

Check if the same lab product or an alternative is used in the 5 most similar protocols
The LV was homogenized and total protein extracted into two fractions: soluble and insoluble. Protein levels were quantified by Bradford assay (BioRad, Hercules, CA). Total protein from the soluble and insoluble fractions was immunoblotted onto nitrocellulose membranes (Bio-Rad). Protein intensities were quantified by densitometry using the IQ-TL image analysis software (GE Healthcare, Waukesha, WI).
Iyer R.P., de Castro Brás L.E., Patterson N.L., Bhowmick M., Flynn E.R., Asher M., Cannon P.L., Deleon-Pennell K.Y., Fields G.B, & Lindsey M.L. (2016). Early matrix metalloproteinase-9 inhibition post-myocardial infarction worsens cardiac dysfunction by delaying inflammation resolution. Journal of molecular and cellular cardiology, 100, 109-117.
+ Open protocol
+ Expand
3

Thbs1 Protein Expression Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunoblotting was performed according to the Guidelines for authors and reviewers on antibody use in physiology studies [5 (link)]. Secretome (20 µl) was separated by 4–12% Criterion™ XT Bis–Tris gels (Bio-Rad) and transferred to nitrocellulose membranes (Bio-Rad). Total protein was stained with MemCode™ Reversible Protein Stain Kit (Thermo Scientific #24580; Waltham, MA). Membranes were blocked for 1 h in 5% milk at room temperature and incubated with a primary antibody against Thbs1 (R&D Systems; #AF3074; 1:500) at 4 °C overnight. Recombinant Thbs1 (R&D Systems #7589-TH; 10 ng) was used as a positive control. Membranes were washed and incubated with secondary antibody (Vector #PI-1000; Malvern, PA, USA) at room temperature for 2 h. Images were detected using ECL Prime Western Blotting Detection Substrate (Amersham; Little Chalfont, UK). Protein expression was analyzed by densitometry using IQ-TL image analysis software (GE Healthcare; Waukesha, WI) and normalized to the total protein.
Mouton A.J., Ma Y., Rivera Gonzalez O.J., Daseke MJ I.I., Flynn E.R., Freeman T.C., Garrett M.R., DeLeon-Pennell K.Y, & Lindsey M.L. (2019). Fibroblast polarization over the myocardial infarction time continuum shifts roles from inflammation to angiogenesis. Basic Research in Cardiology, 114(2), 6.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!