SW480 colorectal cancer cell line (ATCC® CCL-228) was maintained in RPMI640 medium supplemented with 10% FBS and 1% penicillin/streptomycin at 37 °C in atmosphere containing 5% CO2. To overexpress Klf4-GFP and GFP-control in SW480 cell line, cells were transiently transfected with 3 μg plasmid DNA (per well in a six-well plate) using Lipofectamine 2000 reagent (ThermoFisher Scientific) according to manufacturer’s instructions. The cell lysates were collected using Laemmli buffer and subjected to Western blot analysis with the following antibodies: rabbit anti-KLF4 (MBL: PM057), rabbit anti-ZEB1 (Cell Signaling: 3396), rabbit anti-SNAI1 (Cell Signaling: 3879), rabbit anti-SNAI2 (Cell Signaling: 9585), and mouse anti-actin (SigmaAldrich: A1978). Then, they were developed using secondary antibodies goat anti-rabbit HRP-conjugated (JacksonImmuno Research: 111-035-144) and goat anti-mouse HRP conjugated (SigmaAldrich: AP200P), respectively.
Rabbit anti snai1
Manufactured by Cell Signaling Technology
Rabbit anti-SNAI1 is a primary antibody that recognizes the SNAI1 protein. SNAI1 is a transcription factor involved in the regulation of epithelial-mesenchymal transition.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using rabbit anti snai1
1
Overexpression of Klf4 in SW480 Cells
2
Overexpression of Klf4 in SW480 Cells
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SW480 colorectal cancer cell line (ATCC® CCL-228) was maintained in RPMI640 medium supplemented with 10% FBS and 1% penicillin/streptomycin at 37 °C in atmosphere containing 5% CO2. To overexpress Klf4-GFP and GFP-control in SW480 cell line, cells were transiently transfected with 3 μg plasmid DNA (per well in a six-well plate) using Lipofectamine 2000 reagent (ThermoFisher Scientific) according to manufacturer’s instructions. The cell lysates were collected using Laemmli buffer and subjected to Western blot analysis with the following antibodies: rabbit anti-KLF4 (MBL: PM057), rabbit anti-ZEB1 (Cell Signaling: 3396), rabbit anti-SNAI1 (Cell Signaling: 3879), rabbit anti-SNAI2 (Cell Signaling: 9585), and mouse anti-actin (SigmaAldrich: A1978). Then, they were developed using secondary antibodies goat anti-rabbit HRP-conjugated (JacksonImmuno Research: 111-035-144) and goat anti-mouse HRP conjugated (SigmaAldrich: AP200P), respectively.
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