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Brilliant violet 421 conjugated anti mouse cd150

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Brilliant Violet 421 conjugated anti-mouse CD150 is a lab equipment product used for flow cytometry applications. It is a fluorescently labeled antibody that binds to the mouse CD150 cell surface antigen.

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Lab products found in correlation

Rat anti mouse cd16 32 antibody, by BD (4 mentions) 7 aad, by BD (4 mentions) Facscanto, by BD (4 mentions) Pe cy5 conjugated anti mouse cd3e, by BD (2 mentions) Pe cy5 conjugated anti mouse cd8, by Thermo Fisher Scientific (2 mentions) Pe cy5 conjugated anti mouse b220, by Thermo Fisher Scientific (2 mentions) Pe cy5 conjugated anti mouse gr 1, by Thermo Fisher Scientific (2 mentions) Pe conjugated anti mouse sca1, by Thermo Fisher Scientific (2 mentions) Apc conjugated anti mouse ckit, by Thermo Fisher Scientific (2 mentions) Fitc conjugated anti mouse cd48, by Thermo Fisher Scientific (2 mentions) Pe cy5 conjugated anti mouse cd4, by Thermo Fisher Scientific (2 mentions) Ack lysing buffer, by Lonza (2 mentions) Coulter counter, by Beckman Coulter (2 mentions) Pe conjugated anti mouse cd201 epcr, by Thermo Fisher Scientific (2 mentions) Fitc conjugated anti human ki67 antibody, by BD (2 mentions) Apc conjugated anti mouse cd45, by Thermo Fisher Scientific (2 mentions) Cytofix cytoperm fixation permeabilization solution kit, by BD (2 mentions) Pe cy5 conjugated anti mouse cd11b, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Brilliant Violet 421 (66 citations) CD150 (24 citations) Anti-CD150 (8 citations)

4 protocols using brilliant violet 421 conjugated anti mouse cd150

1

Multiparametric Flow Cytometry Analysis of Murine Hematopoietic Cells

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Whole bone marrow (WBM) cells were isolated from femurs and tibias by grinding the bones in HSC buffer. RBCs were lysed using ACK lysing buffer (Lonza). Total number of WBM cells was counted with a Coulter counter (Beckman Coulter). Three million WBM cells were blocked with a rat anti-mouse CD16/32 antibody (BD Pharmingen) and stained with PE-Cy5 conjugated anti-mouse CD3e (clone: 145-2C11), PE-Cy5 conjugated anti-mouse CD4 (clone: GK1.5), PE-Cy5 conjugated anti-mouse CD8 (clone: 53-6.7) PE-Cy5 conjugated anti-mouse B220 (clone: RA3-6B2), PE-Cy5 conjugated PE-Cy5 conjugated anti-mouse CD11b (clone: M1/70), PE-Cy5 conjugated anti-mouse Gr-1 (clone: RB6-8C5), PE-Cy5 conjugated anti-mouse Ter119 (Ly-76), PE conjugated anti-mouse Sca1 (clone:D7), APC conjugated anti-mouse cKit (clone: 2B8), FITC conjugated anti-mouse CD48 (clone: HM48-1) (eBioscience) and Brilliant Violet 421 conjugated anti-mouse CD150 (clone: TC15-12F12.2) antibodies (BioLegend). All antibodies were diluted 1:400. Dead cells were excluded by staining with 7-AAD (BD Pharmingen). Data were collected from 1 million single cells by FACSCanto (BD Pharmingen) and analyzed by FlowJo (Tree Star, Inc) without knowledge of the genotype or treatment by a single observer (CLL).
Lee C.L., Castle K.D., Moding E.J., Blum J.M., Williams N., Luo L., Ma Y., Borst L.B., Kim Y, & Kirsch D.G. (2015). Acute DNA damage activates the tumour suppressor p53 to promote radiation-induced lymphoma. Nature Communications, 6, 8477.
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2

Multicolor Flow Cytometry Analysis of Murine Hematopoietic Stem Cells

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3 × 106 RBC-lysed WBM cells were blocked with a rat anti-mouse CD16/32 antibody (BD Pharmingen) and stained with APC conjugated anti-mouse CD45 (clone: 30-F11), PE conjugated anti-mouse CD201 (EPCR) (clone: eBio1560), APC-eFluor780 conjugated anti-mouse CD48 (clone: HM48-1) (eBioscience) and Brilliant Violet 421 conjugated anti-mouse CD150 (clone: TC15-12F12.2) antibodies (BioLegend). All antibodies were diluted 1:400. Stained cells were fixed in HSC buffer containing 2% paraformaldehyde overnight at 4°C. Fixed WBM cells were permeabilized using HSC buffer containing 0.025% Triton X-100 followed by Cytofix/Cytoperm Fixation/Permeabilization Solution Kit (BD Pharmingen). Permeablized WBM cells were stained with a FITC conjugated anti-human Ki67 antibody (BD Pharmingen) and then resuspend in HSC buffer containing 7AAD (BD Pharmingen). Data were collected from 1 million single cells by FACSCanto (BD Pharmingen) and analyzed by FlowJo (Tree Star, Inc) without knowledge of the genotype or treatment by a single observer (CLL).
Lee C.L., Castle K.D., Moding E.J., Blum J.M., Williams N., Luo L., Ma Y., Borst L.B., Kim Y, & Kirsch D.G. (2015). Acute DNA damage activates the tumour suppressor p53 to promote radiation-induced lymphoma. Nature Communications, 6, 8477.
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3

Murine Hematopoietic Stem Cell Immunophenotyping

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WBM cells were isolated from femurs and tibias by grinding the bones in HSC buffer. RBCs were lysed using ACK lysing buffer (Lonza). Total number of WBM cells was counted with a Coulter counter (Beckman Coulter). Three million WBM cells were blocked with a rat anti-mouse CD16/32 antibody (BD Pharmingen) and stained with PE-Cy5 conjugated anti-mouse CD3e (clone: 145-2C11), PE-Cy5 conjugated anti-mouse CD4 (clone: GK1.5), PE-Cy5 conjugated anti-mouse CD8 (clone: 53-6.7) PE-Cy5 conjugated anti-mouse B220 (clone: RA3-6B2), PE-Cy5 conjugated anti-mouse CD11b (clone: M1/70), PE-Cy5 conjugated anti-mouse Gr-1 (clone: RB6-8C5), PE-Cy5 conjugated anti-mouse Ter119 (Ly-76), PE-conjugated anti-mouse Sca1 (clone:D7), APC conjugated anti-mouse cKit (clone: 2B8), FITC conjugated anti-mouse CD48 (clone: HM48-1; eBioscience) and Brilliant Violet 421 conjugated anti-mouse CD150 (clone: TC15-12F12.2; BioLegend) antibodies. All antibodies were diluted 1:400. Dead cells were excluded by staining with 7AAD (BD Pharmingen). Data were collected from 1 million single cells by FACSCanto (BD Pharmingen) and analysed by FlowJo (Tree Star, Inc.) without knowledge of the genotype or treatment by a single observer (C.L.L.).
Lee C.L., Castle K.D., Moding E.J., Blum J.M., Williams N., Luo L., Ma Y., Borst L.B., Kim Y, & Kirsch D.G. (2015). Acute DNA damage activates the tumour suppressor p53 to promote radiation-induced lymphoma. Nature Communications, 6, 8477.
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4

Immunophenotyping of Hematopoietic Stem Cells

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3 × 106 RBC-lysed WBM cells were blocked with a rat anti-mouse CD16/32 antibody (BD Pharmingen) and stained with APC conjugated anti-mouse CD45 (clone: 30-F11; eBioscience), PE-conjugated anti-mouse CD201 (EPCR; clone: eBio1560; eBioscience), APC-eFluor780 conjugated anti-mouse CD48 (clone: HM48-1; eBioscience) and Brilliant Violet 421 conjugated anti-mouse CD150 (clone: TC15-12F12.2; BioLegend) antibodies. All antibodies were diluted 1:400. Stained cells were fixed in HSC buffer containing 2% paraformaldehyde overnight at 4 °C. Fixed WBM cells were permeabilized using HSC buffer containing 0.025% Triton X-100 followed by Cytofix/Cytoperm Fixation/Permeabilization Solution Kit (BD Pharmingen). Permeablized WBM cells were stained with a FITC conjugated anti-human Ki67 antibody (BD Pharmingen) and then resuspend in HSC buffer containing 7AAD (BD Pharmingen). Data were collected from 1 million single cells by FACSCanto (BD Pharmingen) and analysed by FlowJo (Tree Star, Inc.) without knowledge of the genotype or treatment by a single observer (C.L.L.).
Lee C.L., Castle K.D., Moding E.J., Blum J.M., Williams N., Luo L., Ma Y., Borst L.B., Kim Y, & Kirsch D.G. (2015). Acute DNA damage activates the tumour suppressor p53 to promote radiation-induced lymphoma. Nature Communications, 6, 8477.
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