HEK293 cells expressing WT corin were cultured in 6-well plates at 37°C in the presence of monensin (2 µM). After 48 h, the cells were washed with PBS. The conditioned medium containing recombinant PCSK6 was added to the cell culture together with heparinase (I and III blend), which removes heparan sulfate chains (Sigma H3917, 0.015, 0.15 and 1.5 U/mL) or chondroitinase ABC, which removes chondroitin sulfate chains (Sigma C3667, 1.5 U/mL). After incubation at 37°C for 2 h, the cells were washed and lysed. Corin protein fragments in the cell lysates were examined by SDS-PAGE and Western blotting.
H3917
Manufactured by Merck Group
Sourced in United States
H3917 is a laboratory equipment product manufactured by Merck Group. It serves as a general-purpose measurement tool, but a detailed description cannot be provided while maintaining an unbiased and factual approach.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using h3917
1
Corin regulation by heparan and chondroitin sulfate
2
Endothelial Glycocalyx Degradation Protocols
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The endothelial glycocalyx was degraded by the infusion of hyaluronidase from bovine testes (25 mg/kg/body weight; H3884, Sigma, St. Louis, MO, USA) and a blend of heparinase I and III from Flavobacterium heparinum (1 U/mouse; H3917, Sigma, St. Louis, MO, USA) for 30 min. Heparinase units are given as Sigma units. The enzymes were prepared in phosphate-buffered saline (PBS) and were infused through femoral vein cannulation. The control animals were infused with PBS alone for 30 min. We have successfully demonstrated in a previous study a significant degradation of the mouse retinal glycocalyx following hyaluronidase infusion at a dose of 12 mg/kg [17 (link)].
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