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Transam nfκb transcription factor assay

Manufactured by Active Motif
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The TransAM® NFκB Transcription Factor Assay is a sensitive and specific assay that measures the activation of the NF-κB transcription factor. The assay utilizes a 96-well plate coated with an oligonucleotide containing the NF-κB consensus binding site. Active NF-κB in the sample binds to this oligonucleotide and is detected through the use of a primary antibody specific for the activated form of NF-κB.

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Lab products found in correlation

Ccl11, by R&D Systems (1 mentions) Cxcl2, by R&D Systems (1 mentions) Ccl20, by R&D Systems (1 mentions)

Close spelling variants of this product

TransAM™ NFκB p65 Chemi Transcription Factor Assay Kit

2 protocols using transam nfκb transcription factor assay

1

Measuring NFκB Activation in Breast Cells

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MCF7, BT474, and DCIS.com cells were serum-starved for 24h before treatment with 10nM E and 10nM P. Nuclear extract was collected 48h later using Active Motif’s Nuclear Extract kit (Active Motif 40010), and the TransAM® NFκB Transcription Factor Assay (Active Motif 43296) was used to measure active NFκB p65 within 25μg of nuclear extract following the manufacturer’s recommendations.
Valdez K.E., Elsarraj H.S., Hong Y., Grimm S.L., Ricci L.R., Fan F., Tawfik O., May L., Cusick T., Inciardi M., Redick M., Gatewood J., Winblad O., Hilsenbeck S., Edwards D.P., Hagan C., Godwin A.K., Fabian C, & Behbod F. (2017). NEMO, a transcriptional target of estrogen and progesterone, is linked to tumor suppressor PML in breast cancer. Cancer research, 77(14), 3802-3813.
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2

Lung Inflammation Biomarker Analysis

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A single excised lung lobe from each mouse was snap frozen before homogenisation in buffers and protease inhibitors as recommended in manufacturers instructions. Levels of CCL7 (eBioscience), CCL11, CCL20, CXCL2 (R&D Systems), and phosphorylated-ERK1 were determined in clarified lung lysates by ELISA. Quantification of activate NFκB subunits was performed with a TransAM NFκB Transcription Factor Assay (Active Motif). All concentrations were normalised to lung lobe weight.
Girkin J., Hatchwell L., Foster P., Johnston S.L., Bartlett N., Collison A, & Mattes J. (2015). CCL7 and IRF7 mediate hallmark inflammatory and interferon responses following rhinovirus 1B infection: CCL7 and IRF-7 in the host response to rhinovirus. Journal of immunology (Baltimore, Md. : 1950), 194(10), 4924-4930.
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