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Tissue tek o c t embedding compound

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Tissue-tek O.C.T. embedding compound is a water-soluble, glycol-based medium used for embedding and freezing tissue samples for cryosectioning. It provides a firm, consistent support for the tissue during sectioning and helps prevent freezing artifacts.

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Lab products found in correlation

Axiovert 200m, by Zeiss (3 mentions) Axiovision 4, by Zeiss (3 mentions) Antibody labeling kit, by Thermo Fisher Scientific (2 mentions) Active caspase 3, by BD (2 mentions) E cadherin decma 1, by BioLegend (2 mentions) Vimentin sc 7557, by Santa Cruz Biotechnology (2 mentions) Neu sc 284, by Santa Cruz Biotechnology (2 mentions) Bio spin p30 columns, by Bio-Rad (2 mentions) Cd45 30 f11, by BioLegend (2 mentions)

Close spelling variants of this product

Tissue-Tek O.C.T. (24 citations) Tissue-Tek (11 citations) Tissue-Tek O.C.T Compound embedding medium (1 citations)

3 protocols using tissue tek o c t embedding compound

1

Comprehensive Histological Profiling of Tumor Metastases

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The harvested primary tumors and PBS-perfused lungs bearing metastases were fixed in 4% paraformaldehyde overnight, followed by 30% sucrose for 2 days, and then embedded in Tissue-tek O.C.T. embedding compound (Electron Microscopy Sciences). Serial sections (10µm, at least 10 sections) were prepared for histological analysis by Hematoxylin & Eosin (H&E) staining, and immunofluorescent staining following standardized protocols.
Primary antibodies used in this study include CD45 (30-F11, BioLegend), E-cadherin (DECMA-1, BioLegend), vimentin (sc-7557, Santa Cruz), PyMT (ab15085, Abcam), Neu (sc-284, Santa Cruz), Ki67 (ab15580, Abcam), and Active Caspase-3 (C92–605, BD Pharmingen). Primary antibodies were directly conjugated to Alexa Fluor 647 using an antibody labeling kit (Invitrogen) performed as per manufacturer’s instructions and purified over BioSpin P30 columns (Bio-Rad). GFP+ and RFP+ cells were detected by inherent fluorescence.
Fluorescent images were obtained using a computerized Zeiss fluorescent microscope (Axiovert 200M), fitted with an apotome and an HRM camera. Images were analyzed using Axiovision 4.6 software (Carl Zeiss Inc.).
Fischer K.R., Durrans A., Lee S., Sheng J., Li F., Wong S., Choi H., El Rayes T., Ryu S., Troeger J., Schwabe R.F., Vahdat L.T., Altorki N.K., Mittal V, & Gao D. (2015). EMT is not required for lung metastasis but contributes to chemoresistance. Nature, 527(7579), 472-476.
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2

Comprehensive Histological Profiling of Tumor Metastases

Check if the same lab product or an alternative is used in the 5 most similar protocols
The harvested primary tumors and PBS-perfused lungs bearing metastases were fixed in 4% paraformaldehyde overnight, followed by 30% sucrose for 2 days, and then embedded in Tissue-tek O.C.T. embedding compound (Electron Microscopy Sciences). Serial sections (10µm, at least 10 sections) were prepared for histological analysis by Hematoxylin & Eosin (H&E) staining, and immunofluorescent staining following standardized protocols.
Primary antibodies used in this study include CD45 (30-F11, BioLegend), E-cadherin (DECMA-1, BioLegend), vimentin (sc-7557, Santa Cruz), PyMT (ab15085, Abcam), Neu (sc-284, Santa Cruz), Ki67 (ab15580, Abcam), and Active Caspase-3 (C92–605, BD Pharmingen). Primary antibodies were directly conjugated to Alexa Fluor 647 using an antibody labeling kit (Invitrogen) performed as per manufacturer’s instructions and purified over BioSpin P30 columns (Bio-Rad). GFP+ and RFP+ cells were detected by inherent fluorescence.
Fluorescent images were obtained using a computerized Zeiss fluorescent microscope (Axiovert 200M), fitted with an apotome and an HRM camera. Images were analyzed using Axiovision 4.6 software (Carl Zeiss Inc.).
Fischer K.R., Durrans A., Lee S., Sheng J., Li F., Wong S., Choi H., El Rayes T., Ryu S., Troeger J., Schwabe R.F., Vahdat L.T., Altorki N.K., Mittal V, & Gao D. (2015). EMT is not required for lung metastasis but contributes to chemoresistance. Nature, 527(7579), 472-476.
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3

Histological Analysis of Tumor and Metastatic Tissues

Check if the same lab product or an alternative is used in the 5 most similar protocols
Harvested primary tumors and PBS-perfused lungs bearing metastases were fixed in 4% paraformaldehyde overnight, followed by 30% sucrose for 2 days, and then embedded in Tissue-tek O.C.T embedding compound (Electron Microscopy Sciences). Serial sections (10mm, at least 10 sections) were prepared for histological analysis by hematoxylin and eosin (H&E) staining, and immunofluorescent staining following standardized protocols. Fluorescent images were obtained using a computerized Zeiss fluorescent microscope (Axiovert 200M), with an apotome and HRM camera. Images were analyzed using Axiovision 4.6 software (Carl Zeiss).
Yomtoubian S., Lee S.B., Verma A., Izzo F., Markowitz G., Choi H., Cerchietti L., Vahdat L., Brown K.A., Andreopoulou E., Elemento O., Chang J., Inghirami G., Gao D., Ryu S, & Mittal V. (2020). Inhibition of EZH2 Catalytic Activity Selectively Targets a Metastatic Subpopulation in Triple-Negative Breast Cancer. Cell reports, 30(3).
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