Balb cj

All procedures performed on animals were approved by Stanford University's Institutional Animal Care and Use Committee and were within the guidelines of humane care of laboratory animals (Protocol #27293). Both control (n=10) and aGVHD mice (n=11) were generated as previously described (11 (link),24 (link),25 (link)). Briefly, recipient 8-16 week old female BALB/c mice (Balb/cJ; 18-22 g; Jackson Laboratories) were irradiated with 800 cGy in two split doses on day 0 with an electron linear accelerator. T cell-depleted bone marrow (TCD-BM) cells were obtained from wild-type 8-16 week old female C57Bl/6 donor mice (control mice; C57Bl/6J; 18-22 g; Jackson Laboratories). Donor T cells were obtained from the spleens of firefly luciferase (Luc⁺) 8-16 week old female C57Bl/6-L2G85 (18-22 g) donor mice (11 (link),26 (link)). T cell depletion and donor T cell selection were performed using CD4 and CD8 magnetic beads (Miltenyi Biotec). All mice received intravenous injection of 5×10⁶ TCD-BM cells to initiate hematopoietic reconstitution. For aGVHD mice, they also received 1×10⁶ Luc⁺ T cells co-injected with the TCD-BM cells. For visualization of donor derived T cells in immunofluorescence experiments, 8-16 week old female Luc⁺gfp⁺ C57Bl/6 mice (18-22 g) were used as the T cell donor (27 (link)).

Adult C57BL/6J, BALB/cJ, B6.129S2-Igh-6^tm1Cgn (µMT), C.129S7(B6)-Rag1^tm1Mom/J (Rag−/−), and C57BL/6-Tg(IghelMD4)4Ccg/J (MD4) mice were purchased from The Jackson Laboratory. C.129(B6)-IgH-JhD^tm1Dhu (Jh), and C.B-Igh-1^b/ICR Tac-Prkdc^Scid (SCID) mice were purchased from Taconic. Mice doubly deficient in activation-induced deaminase (AID) and µS were crossed in the lab of Hidde Ploegh (AID-µS^−/−) and fail to secrete antibody but have a polyclonal BCR repertoire (18 (link)). All experimental mice were housed in the Veterans Administration (VA) Medical Center veterinary medical unit or University of Kentucky Division of Laboratory Animal Resources units in sanitized cages, and given food and water ad libitum. PC organisms were maintained in a colony of Rag2^−/− mice (originally from Jackson Laboratory) as a source for all infections. All procedures were approved by the Lexington VA or University of Kentucky Institutional Animal Care and Use Committees.

All experimental procedures were performed under approval by Washington University’s Animal Studies Committee. C57BL/6J, BALB/cJ, BALB/cBYJ (Acads^−/−), Foxo1-floxed, and Foxo3-floxed mice were purchased from Jackson Laboratories and bred in house. All experiments used littermate controls.

Mice were maintained at the University of California, Berkeley. C57BL/6J, CD45.1-congenic (B6.SJL-Ptprc^aPepc^b/BoyJ), Rag2^−/−, Rag2^−/−Il2rg^−/−, Ifnar1^−/− (all on the B6 background), and BALB/cJ mice were purchased from the Jackson Laboratory. Ncr1^iCre and Sting^gt/gt mice on the B6 background were generous gifts from Eric Vivier and Russell Vance, respectively. NK-DTA mice were generated by breeding Ncr1^iCre mice to B6-Rosa26^LSL-DTA mice (Jackson Laboratories). Ncr1^iCre/+, Ifnar1^fl/fl and CD11c-Cre, Ifnar1^fl/fl mice, all on the B6 background, were generated by breeding Ncr1^iCre and CD11c (Itgax)-Cre-eGFP (Jackson Laboratories) mice to Ifnar1^fl/fl mice (Jackson Laboratories). All mice used were aged 8–30 weeks. All experiments were approved by the UC Berkeley Animal Care and Use Committee.